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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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November 2007 Volume 18 Issue 5

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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November 2007 Volume 18 Issue 5

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Article

Influence of cytological stains on comparative genomic hybridization analysis for DNA extracted from cytological smears

  • Authors:
    • Shigeto Kawauchi
    • Sayaka Sugimoto
    • Xu-Ping Liu
    • Kenzo Ikemoto
    • Tomoko Furuya
    • Mutsuo Takahashi
    • Kohsuke Sasaki
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Yamaguchi University School of Medicine, Yamaguchi 755-8505, Japan. shig@yamaguchi-u.ac.jp
  • Pages: 1219-1223
    |
    Published online on: November 1, 2007
       https://doi.org/10.3892/or.18.5.1219
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Abstract

In the present study, we investigated the influence of cytological stains in analyzing DNA extracted from cytological slides by comparative genomic hybridization (CGH). Multiple imprint cytological slides were prepared for fresh-frozen breast cancer tissue samples and the slides were stained by three staining methods for each sample. Under microscopic observation, cancer cells were selectively microdissected from the slides and forwarded to DNA extraction, whole genome amplification, and CGH analysis. CGH was successfully performed for all methylgreen-stained and May-Grunwald-Giemsa (MGG)-stained cytological smear slides, but for two Papanicolaou (PAP)-stained slides. The number of chromosomal imbalances detected were 5-10 in methylgreen-stained slides and 5-9 in MGG-stained slides. The chromosomal imbalances resemble each other between methylgreen-stained and MGG-stained slides. The present study indicates that the MGG stain is preferred to the PAP stain for the purpose of cytogenetical analysis by CGH for DNA extracted from cytological smear slides.

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Copy and paste a formatted citation
Spandidos Publications style
Kawauchi S, Sugimoto S, Liu X, Ikemoto K, Furuya T, Takahashi M and Sasaki K: Influence of cytological stains on comparative genomic hybridization analysis for DNA extracted from cytological smears. Oncol Rep 18: 1219-1223, 2007.
APA
Kawauchi, S., Sugimoto, S., Liu, X., Ikemoto, K., Furuya, T., Takahashi, M., & Sasaki, K. (2007). Influence of cytological stains on comparative genomic hybridization analysis for DNA extracted from cytological smears. Oncology Reports, 18, 1219-1223. https://doi.org/10.3892/or.18.5.1219
MLA
Kawauchi, S., Sugimoto, S., Liu, X., Ikemoto, K., Furuya, T., Takahashi, M., Sasaki, K."Influence of cytological stains on comparative genomic hybridization analysis for DNA extracted from cytological smears". Oncology Reports 18.5 (2007): 1219-1223.
Chicago
Kawauchi, S., Sugimoto, S., Liu, X., Ikemoto, K., Furuya, T., Takahashi, M., Sasaki, K."Influence of cytological stains on comparative genomic hybridization analysis for DNA extracted from cytological smears". Oncology Reports 18, no. 5 (2007): 1219-1223. https://doi.org/10.3892/or.18.5.1219
Copy and paste a formatted citation
x
Spandidos Publications style
Kawauchi S, Sugimoto S, Liu X, Ikemoto K, Furuya T, Takahashi M and Sasaki K: Influence of cytological stains on comparative genomic hybridization analysis for DNA extracted from cytological smears. Oncol Rep 18: 1219-1223, 2007.
APA
Kawauchi, S., Sugimoto, S., Liu, X., Ikemoto, K., Furuya, T., Takahashi, M., & Sasaki, K. (2007). Influence of cytological stains on comparative genomic hybridization analysis for DNA extracted from cytological smears. Oncology Reports, 18, 1219-1223. https://doi.org/10.3892/or.18.5.1219
MLA
Kawauchi, S., Sugimoto, S., Liu, X., Ikemoto, K., Furuya, T., Takahashi, M., Sasaki, K."Influence of cytological stains on comparative genomic hybridization analysis for DNA extracted from cytological smears". Oncology Reports 18.5 (2007): 1219-1223.
Chicago
Kawauchi, S., Sugimoto, S., Liu, X., Ikemoto, K., Furuya, T., Takahashi, M., Sasaki, K."Influence of cytological stains on comparative genomic hybridization analysis for DNA extracted from cytological smears". Oncology Reports 18, no. 5 (2007): 1219-1223. https://doi.org/10.3892/or.18.5.1219
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