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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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September 2011 Volume 26 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

A fully integrated, automated and rapid detection system for KRAS mutations

  • Authors:
    • Norio Ureshino
    • Naoko Sueoka-Aragane
    • Tomomi Nakamura
    • Akemi Sato
    • Kazutoshi Komiya
    • Kentaro Iwanaga
    • Masahiro Mitsuoka
    • Yuji Takeda
    • Shinichiro Hayashi
    • Eisaburo Sueoka
    • Shinya Kimura

  • View Affiliations / Copyright

    Affiliations: Division of Hematology, Respiratory Medicine and Oncology, Department of Internal Medicine, Faculty of Medicine, Saga University, Saga 849-8501, Japan, Division of Hematology, Respiratory Medicine and Oncology, Department of Internal Medicine, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga 849-8501, Japan
  • Pages: 609-613
    |
    Published online on: June 7, 2011
       https://doi.org/10.3892/or.2011.1341
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Abstract

KRAS mutations are detected in tumors of various organs, and they are also markers of resistance for epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors and monoclonal antibodies against the EGFR. Thus, the accurate and rapid detection of KRAS mutations is crucial, not only for screening, but also for the prediction of the efficacy of molecular-targeted therapy. The aim of the present study was to establish a novel automated detection system for KRAS mutations. One hundred and thirty-six lung adenocarcinoma patients were genotyped for KRAS mutations with both the conventional direct sequence (DS) method and with the newly developed quenching probe (QP) method that obtains data automatically within 60 min. The detection limit of the QP method using a control plasmid containing the KRAS mutation was 50 copies, and 10% mutant plasmid was detected in the mixture of wild-type and mutants. The results obtained by the QP and DS methods were identical in all but two of the 136 cases. The two differentially identified samples, which consisted of substantially fewer lung cancer cells, were positive according to the QP method but negative as determined by DS for KRAS mutations. These findings characterize the QP method as an accurate and rapid detection system for KRAS mutations.

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Copy and paste a formatted citation
Spandidos Publications style
Ureshino N, Sueoka-Aragane N, Nakamura T, Sato A, Komiya K, Iwanaga K, Mitsuoka M, Takeda Y, Hayashi S, Sueoka E, Sueoka E, et al: A fully integrated, automated and rapid detection system for KRAS mutations. Oncol Rep 26: 609-613, 2011.
APA
Ureshino, N., Sueoka-Aragane, N., Nakamura, T., Sato, A., Komiya, K., Iwanaga, K. ... Kimura, S. (2011). A fully integrated, automated and rapid detection system for KRAS mutations. Oncology Reports, 26, 609-613. https://doi.org/10.3892/or.2011.1341
MLA
Ureshino, N., Sueoka-Aragane, N., Nakamura, T., Sato, A., Komiya, K., Iwanaga, K., Mitsuoka, M., Takeda, Y., Hayashi, S., Sueoka, E., Kimura, S."A fully integrated, automated and rapid detection system for KRAS mutations". Oncology Reports 26.3 (2011): 609-613.
Chicago
Ureshino, N., Sueoka-Aragane, N., Nakamura, T., Sato, A., Komiya, K., Iwanaga, K., Mitsuoka, M., Takeda, Y., Hayashi, S., Sueoka, E., Kimura, S."A fully integrated, automated and rapid detection system for KRAS mutations". Oncology Reports 26, no. 3 (2011): 609-613. https://doi.org/10.3892/or.2011.1341
Copy and paste a formatted citation
x
Spandidos Publications style
Ureshino N, Sueoka-Aragane N, Nakamura T, Sato A, Komiya K, Iwanaga K, Mitsuoka M, Takeda Y, Hayashi S, Sueoka E, Sueoka E, et al: A fully integrated, automated and rapid detection system for KRAS mutations. Oncol Rep 26: 609-613, 2011.
APA
Ureshino, N., Sueoka-Aragane, N., Nakamura, T., Sato, A., Komiya, K., Iwanaga, K. ... Kimura, S. (2011). A fully integrated, automated and rapid detection system for KRAS mutations. Oncology Reports, 26, 609-613. https://doi.org/10.3892/or.2011.1341
MLA
Ureshino, N., Sueoka-Aragane, N., Nakamura, T., Sato, A., Komiya, K., Iwanaga, K., Mitsuoka, M., Takeda, Y., Hayashi, S., Sueoka, E., Kimura, S."A fully integrated, automated and rapid detection system for KRAS mutations". Oncology Reports 26.3 (2011): 609-613.
Chicago
Ureshino, N., Sueoka-Aragane, N., Nakamura, T., Sato, A., Komiya, K., Iwanaga, K., Mitsuoka, M., Takeda, Y., Hayashi, S., Sueoka, E., Kimura, S."A fully integrated, automated and rapid detection system for KRAS mutations". Oncology Reports 26, no. 3 (2011): 609-613. https://doi.org/10.3892/or.2011.1341
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