Induction of apoptosis by ethanol extract of Prunus mume in U937 human leukemia cells through activation of caspases

  • Authors:
    • Cheol Park
    • Cheng-Yun Jin
    • Gi-Young Kim
    • Yong Kee Jeong
    • Wun-Jae Kim
    • Yung Hyun Choi
  • View Affiliations

  • Published online on: June 27, 2011     https://doi.org/10.3892/or.2011.1363
  • Pages: 987-993
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Abstract

Prunus mume (P. mume), a traditional drug and health food in Korea, Japan and China, possesses various pharmacological activities that include a potential source of free radical scavenging, anti-viral, anti-microbial, anti-inflammatory and anti-cancer activities. However, the cellular and molecular mechanisms of apoptosis induction by P. mume in human cancer cells are poorly understood. In the present study, we conducted an investigation of the pro-apoptotic effects of an ethanol extract of P. mume (EEPM) in U937 human leukemia cells. Exposure to EEPM was found to result in a concentration-dependent growth inhibition by induction of apoptosis. Induction of apoptotic cell death of U937 cells by EEPM showed a correlation with the down-regulation of members of the inhibitor of apoptosis protein (IAP) family, including X-linked inhibitor of apoptosis protein (XIAP) and survivin, and anti-apoptotic Bcl-2, up-regulation of FasL, and cleavage of Bic. EEPM treatment induced proteo­lytic activation of caspase-3, -8 and -9, and degradation of caspase-3 substrate proteins, including poly(ADP-ribose) polymerase (PARP) and β-catenin. In addition, apoptotic cell death induced by EEPM was significantly inhibited by z-DEVD-fmk, a caspase-3-specific inhibitor, which demonstrated the important role played by caspase-3 in the process. Taken together, these findings suggest that EEPM may be a potential chemotherapeutic agent for use in the control of human leukemia U937 cells and that further studies are needed for the identification of the active compounds.

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October 2011
Volume 26 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Park C, Jin C, Kim G, Jeong YK, Kim W and Choi YH: Induction of apoptosis by ethanol extract of Prunus mume in U937 human leukemia cells through activation of caspases. Oncol Rep 26: 987-993, 2011.
APA
Park, C., Jin, C., Kim, G., Jeong, Y.K., Kim, W., & Choi, Y.H. (2011). Induction of apoptosis by ethanol extract of Prunus mume in U937 human leukemia cells through activation of caspases. Oncology Reports, 26, 987-993. https://doi.org/10.3892/or.2011.1363
MLA
Park, C., Jin, C., Kim, G., Jeong, Y. K., Kim, W., Choi, Y. H."Induction of apoptosis by ethanol extract of Prunus mume in U937 human leukemia cells through activation of caspases". Oncology Reports 26.4 (2011): 987-993.
Chicago
Park, C., Jin, C., Kim, G., Jeong, Y. K., Kim, W., Choi, Y. H."Induction of apoptosis by ethanol extract of Prunus mume in U937 human leukemia cells through activation of caspases". Oncology Reports 26, no. 4 (2011): 987-993. https://doi.org/10.3892/or.2011.1363