Notch1 is involved in migration and invasion of human breast cancer cells

  • Authors:
    • Jing Wang
    • Li Fu
    • Feng Gu
    • Yongjie Ma
  • View Affiliations

  • Published online on: July 22, 2011     https://doi.org/10.3892/or.2011.1399
  • Pages: 1295-1303
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Abstract

The Notch pathway displays several functions related to tumor progression. Breast carcinomas commonly express Notch1, Notch2, Notch3 and Notch4 at variable levels and these are mainly involved in differentiation, proliferation and survival. Notch1 can also induce the invasion of breast cancer cells. However, the precise role and mechanism of Notch1 in tumor invasion remains unclear. In this report, we used small interference RNA technology to knock down the expression of Notch1, resulting in reduced migration and invasion of breast cancer cells. Meanwhile, F-actin polymerization, which is essential for cellular generation of the forces needed for motility, was also impaired in Notch1 knockdown cells. We further investigated the expression of extracellular matrix metalloproteinase inducer (EMMPRIN), matrix metalloproteases-2 (MMP-2) and MMP-9, and found that the expression of functional EMMPRIN and MMP-2 was significantly decreased in Notch1 knockdown cells, while the expression of MMP-9 was constant. Additionally, the silencing of Notch1 expression likewise impaired cell-to-matrix and cell-to-cell adhesion. Western blotting results showed that reduction of Notch1 levels impacted the phosphorylation of PAK, phosphorylation of Akt, phosphorylation of FAK, the phosphorylation of integrin β1, ICAM-1 and β-catenin. Collectively, these findings suggest that targeting Notch1 has important therapeutic value in breast cancer.

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November 2011
Volume 26 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wang J, Fu L, Gu F and Ma Y: Notch1 is involved in migration and invasion of human breast cancer cells. Oncol Rep 26: 1295-1303, 2011
APA
Wang, J., Fu, L., Gu, F., & Ma, Y. (2011). Notch1 is involved in migration and invasion of human breast cancer cells. Oncology Reports, 26, 1295-1303. https://doi.org/10.3892/or.2011.1399
MLA
Wang, J., Fu, L., Gu, F., Ma, Y."Notch1 is involved in migration and invasion of human breast cancer cells". Oncology Reports 26.5 (2011): 1295-1303.
Chicago
Wang, J., Fu, L., Gu, F., Ma, Y."Notch1 is involved in migration and invasion of human breast cancer cells". Oncology Reports 26, no. 5 (2011): 1295-1303. https://doi.org/10.3892/or.2011.1399