Epigenetic silencing of microRNA-373 plays an important role in regulating cell proliferation in colon cancer

  • Authors:
    • Takeshi Tanaka
    • Makoto Arai
    • Shuang Wu
    • Tatsuo Kanda
    • Hideaki Miyauchi
    • Fumio Imazeki
    • Hisahiro Matsubara
    • Osamu Yokosuka
  • View Affiliations

  • Published online on: July 22, 2011     https://doi.org/10.3892/or.2011.1401
  • Pages:1329-1335
0

Abstract

microRNAs (miRNA) are non-coding RNAs that negatively control gene expression by cleaving or inhibiting the translation of target gene mRNAs. We used a microarray-based transcriptomic analysis to identify miRNA expression levels that changed in response to epigenetic factors. Specifically, we searched for increased expression of miRNAs prepared from colon cancer cell line DLD-1 after a 96-h treatment with 5 µM of 5-aza-2'-deoxycytidine (DAC). Among those identified, transient transfection of miRNA hsa-miR-373 resulted in cytostasis. In addition, bisulfate sequence analysis of the promoter regions of these miRNAs showed aberrant methylation in the cancer cells. In clinical colon samples, hsa-miR-373 was down-regulated in colon cancers (29/40, 72.5%) relative to control samples, whereas the purported oncogene RAB22A (a target gene of hsa-miR-373) was up-regulated (24/40, 60%). Using methylation-specific PCR, we also observed aberrant methylation of hsa-miR-373 in colon cancers (35/40, 87.5%) relative to controls (8/40, 20%). Based on these results, we conclude that expression of hsa-miR-373 is down-regulated by aberrant methylation in colon cancer and that this miRNA may function by regulating expression of the oncogene RAB22A.

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November 2011
Volume 26 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2016 Impact Factor: 2.662
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Copy and paste a formatted citation
APA
Tanaka, T., Arai, M., Wu, S., Kanda, T., Miyauchi, H., Imazeki, F. ... Yokosuka, O. (2011). Epigenetic silencing of microRNA-373 plays an important role in regulating cell proliferation in colon cancer. Oncology Reports, 26, 1329-1335. https://doi.org/10.3892/or.2011.1401
MLA
Tanaka, T., Arai, M., Wu, S., Kanda, T., Miyauchi, H., Imazeki, F., Matsubara, H., Yokosuka, O."Epigenetic silencing of microRNA-373 plays an important role in regulating cell proliferation in colon cancer". Oncology Reports 26.5 (2011): 1329-1335.
Chicago
Tanaka, T., Arai, M., Wu, S., Kanda, T., Miyauchi, H., Imazeki, F., Matsubara, H., Yokosuka, O."Epigenetic silencing of microRNA-373 plays an important role in regulating cell proliferation in colon cancer". Oncology Reports 26, no. 5 (2011): 1329-1335. https://doi.org/10.3892/or.2011.1401