Expression and function of miR-27b in human glioma

  • Authors:
    • Lingchao Chen
    • Huibing Li
    • Lei Han
    • Kailiang Zhang
    • Guangxiu Wang
    • Yongzhi Wang
    • Yanwei Liu
    • Yongri Zheng
    • Tao Jiang
    • Peiyu Pu
    • Chuanlu Jiang
    • Chunsheng Kang
  • View Affiliations

  • Published online on: September 14, 2011     https://doi.org/10.3892/or.2011.1458
  • Pages: 1617-1621
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Abstract

Our previous miRNAs profiling study showed that miR-27b was up-regulated in glioma cells compared with H4 low grade astrocytoma cells. However, the main function of miR-27b in glioma in not known yet. The aim of this study was to investigate the expression and function of miR-27b in the pathogenesis of glioma. Real-time PCR showed that miR-27b was up-regulated in glioma samples and glioma cells. Down-regulation of miR-27b triggered growth inhibition, induced apoptosis and inhibited invasion in glioma cells. Furthermore, TOPflash luciferase activity was decreased significantly, while FOPflash luciferase did not change significantly. In addition, Western blot assay showed that STAT3, c-myc and cyclin D1 were knocked down after treatment with miR-27b inhibitor. These findings suggest that aberrantly up-regulated miR-27b may be one of the critical factors that contribute to malignancy in human gliomas.

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December 2011
Volume 26 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Chen L, Li H, Han L, Zhang K, Wang G, Wang Y, Liu Y, Zheng Y, Jiang T, Pu P, Pu P, et al: Expression and function of miR-27b in human glioma. Oncol Rep 26: 1617-1621, 2011
APA
Chen, L., Li, H., Han, L., Zhang, K., Wang, G., Wang, Y. ... Kang, C. (2011). Expression and function of miR-27b in human glioma. Oncology Reports, 26, 1617-1621. https://doi.org/10.3892/or.2011.1458
MLA
Chen, L., Li, H., Han, L., Zhang, K., Wang, G., Wang, Y., Liu, Y., Zheng, Y., Jiang, T., Pu, P., Jiang, C., Kang, C."Expression and function of miR-27b in human glioma". Oncology Reports 26.6 (2011): 1617-1621.
Chicago
Chen, L., Li, H., Han, L., Zhang, K., Wang, G., Wang, Y., Liu, Y., Zheng, Y., Jiang, T., Pu, P., Jiang, C., Kang, C."Expression and function of miR-27b in human glioma". Oncology Reports 26, no. 6 (2011): 1617-1621. https://doi.org/10.3892/or.2011.1458