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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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January 2012 Volume 27 Issue 1

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Short interference RNA introduced into cultured cells with diagnostic ultrasound

  • Authors:
    • Minoru Tomizawa
    • Fuminobu Shinozaki
    • Takao Sugiyama
    • Shigenori Yamamoto
    • Makoto Sueishi
    • Takanobu Yoshida

  • View Affiliations / Copyright

    Affiliations: Department of Gastro­enterology, National Hospital Organization Shimoshizu Hospital, 934-5 Shikawatashi, Yotsukaido City, Chiba 284-0003, Japan
  • Pages: 65-68
    |
    Published online on: October 13, 2011
       https://doi.org/10.3892/or.2011.1506
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Abstract

Diagnostic ultrasound (US) is safe. Short interference (si) RNA of Frizzled (Fz)-9 suppresses proliferation of hepatocellular carcinoma cells. siRNA was introduced into Hep3B cells, a human hepatocellular carcinoma cell line, with transfection or US upward to culture plates at mechanical index (MI) = 0.4 or 0.8. siRNA of Fz9 was introduced for western blot analysis and cell proliferation assay. siRNA of Fz9 suppressed cell proliferation to 32.8±13.6% at 200 nM (P<0.0001) with transfection. With US, cell proliferation decreased to 36.9±15.1% at 200 nM (P=0.039) at 0.4 of MI while 49.2±12.0% at 200 nM (P=0.041) at MI=0.8. Western blot analysis showed that 200 nM of siRNA decreased the expression of Fz9 with US at MI=0.4 and MI=0.8. No change was seen in cell proliferation between transfection and US. siRNA was introduced into cultured cells and functioned with US.

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Copy and paste a formatted citation
Spandidos Publications style
Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M and Yoshida T: Short interference RNA introduced into cultured cells with diagnostic ultrasound. Oncol Rep 27: 65-68, 2012.
APA
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., & Yoshida, T. (2012). Short interference RNA introduced into cultured cells with diagnostic ultrasound. Oncology Reports, 27, 65-68. https://doi.org/10.3892/or.2011.1506
MLA
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T."Short interference RNA introduced into cultured cells with diagnostic ultrasound". Oncology Reports 27.1 (2012): 65-68.
Chicago
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T."Short interference RNA introduced into cultured cells with diagnostic ultrasound". Oncology Reports 27, no. 1 (2012): 65-68. https://doi.org/10.3892/or.2011.1506
Copy and paste a formatted citation
x
Spandidos Publications style
Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M and Yoshida T: Short interference RNA introduced into cultured cells with diagnostic ultrasound. Oncol Rep 27: 65-68, 2012.
APA
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., & Yoshida, T. (2012). Short interference RNA introduced into cultured cells with diagnostic ultrasound. Oncology Reports, 27, 65-68. https://doi.org/10.3892/or.2011.1506
MLA
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T."Short interference RNA introduced into cultured cells with diagnostic ultrasound". Oncology Reports 27.1 (2012): 65-68.
Chicago
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T."Short interference RNA introduced into cultured cells with diagnostic ultrasound". Oncology Reports 27, no. 1 (2012): 65-68. https://doi.org/10.3892/or.2011.1506
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