Open Access

Survivin deficiency induces apoptosis and cell cycle arrest in HepG2 hepatocellular carcinoma cells

  • Authors:
    • Dejian Dai
    • Yunjia Liang
    • Zhihua Xie
    • Jun Fu
    • Yuhao Zhang
    • Zhijin Zhang
  • View Affiliations

  • Published online on: November 10, 2011     https://doi.org/10.3892/or.2011.1544
  • Pages: 621-627
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Abstract

The postulated dual roles of survivin as an anti-apoptotic factor and a mitotic inducer have placed this factor in the spotlight of cancer research. The purpose of this study was to investigate whether survivin might connect the cell cycle with apoptosis. Here, by simultaneously monitoring survivin deficiency-induced morphological changes of HepG2 cells using time-lapse imaging as well as determining apoptosis progression, we observed synchronized defective mitosis characterized by multinucleated and polyploid cells and cell cycle arrest at S phase or G2/M phase followed by apoptosis, the processes of which depended on the simultaneous destruction of specialized subcellular compartments of survivin and activation of caspase-3-like protease. These findings showed that the survivin protein acted as mitotic regulator and apoptosis inhibitor, but may also possess the role of a bridge in integrating apoptosis and cell division. An essential prerequisite of this pathway was the specialized subcellular localization of survivin. The overexpression of survivin was required to maintain cell viability and proper cell cycle transitions, and to preserve genetic fidelity during cell division in HepG2 cells.

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March 2012
Volume 27 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Dai D, Liang Y, Xie Z, Fu J, Zhang Y and Zhang Z: Survivin deficiency induces apoptosis and cell cycle arrest in HepG2 hepatocellular carcinoma cells. Oncol Rep 27: 621-627, 2012.
APA
Dai, D., Liang, Y., Xie, Z., Fu, J., Zhang, Y., & Zhang, Z. (2012). Survivin deficiency induces apoptosis and cell cycle arrest in HepG2 hepatocellular carcinoma cells. Oncology Reports, 27, 621-627. https://doi.org/10.3892/or.2011.1544
MLA
Dai, D., Liang, Y., Xie, Z., Fu, J., Zhang, Y., Zhang, Z."Survivin deficiency induces apoptosis and cell cycle arrest in HepG2 hepatocellular carcinoma cells". Oncology Reports 27.3 (2012): 621-627.
Chicago
Dai, D., Liang, Y., Xie, Z., Fu, J., Zhang, Y., Zhang, Z."Survivin deficiency induces apoptosis and cell cycle arrest in HepG2 hepatocellular carcinoma cells". Oncology Reports 27, no. 3 (2012): 621-627. https://doi.org/10.3892/or.2011.1544