Protective effects of thioredoxin-mediated p53 activation in response to mild hyperthermia

  • Authors:
    • Hwa Jin Jung
    • Young Rok Seo
  • View Affiliations

  • Published online on: November 28, 2011     https://doi.org/10.3892/or.2011.1564
  • Pages: 650-656
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Abstract

Recently, mild hyperthermia was shown to induce cell cycle arrest at the G2/M phase transition without leading to DNA damage. The mechanism of this regulation has not yet been elucidated, although p53 has been shown to be activated in response to mild hyperthermia. Here, we report the role of thioredoxin (TXN) in mild hyperthermia-induced cellular responses. Our data showed that the protein levels of p53 and its downstream gene, Gadd45a, which is an indicator of G2/M arrest, were significantly decreased in TXN siRNA-treated cells under conditions of mild hyperthermia (41˚C, 60 min) as compared to TXN wild-type cells, implying that TXN might play an important role in mild hyperthermia-induced G2/M arrest via p53 and Gadd45a activation. Furthermore, the release of cyclin-dependent kinase Cdc2, known to be regulated by Gadd45a under G2/M arrest, was inhibited from the nucleus for arrest in the G2/M phase in TXN downregulated cells under mild hyperthermia. We suggest that G2/M arrest mediated via the TXN-modulated p53 in response to mild hyperthermia may provide critical insight into the clinical use of mild hyperthermia to induce an adaptive response against genotoxic stresses.

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March 2012
Volume 27 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Jung HJ and Jung HJ: Protective effects of thioredoxin-mediated p53 activation in response to mild hyperthermia. Oncol Rep 27: 650-656, 2012
APA
Jung, H.J., & Jung, H.J. (2012). Protective effects of thioredoxin-mediated p53 activation in response to mild hyperthermia. Oncology Reports, 27, 650-656. https://doi.org/10.3892/or.2011.1564
MLA
Jung, H. J., Seo, Y. R."Protective effects of thioredoxin-mediated p53 activation in response to mild hyperthermia". Oncology Reports 27.3 (2012): 650-656.
Chicago
Jung, H. J., Seo, Y. R."Protective effects of thioredoxin-mediated p53 activation in response to mild hyperthermia". Oncology Reports 27, no. 3 (2012): 650-656. https://doi.org/10.3892/or.2011.1564