Identification of aberrantly expressed miRNAs in rectal cancer

Li,Xinhua; Zhang,Guiying; Luo,Feijun; Ruan,Jinde; Huang,Damao; Feng,Deyun; Xiao,Desheng; Zeng,Zhijun; Chen,Xiong; Wu,Wei

doi:10.3892/or.2012.1769

Identification of aberrantly expressed miRNAs in rectal cancer

Authors:
- Xinhua Li
- Guiying Zhang
- Feijun Luo
- Jinde Ruan
- Damao Huang
- Deyun Feng
- Desheng Xiao
- Zhijun Zeng
- Xiong Chen
- Wei Wu
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Affiliations: Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, P.R. China, Cambridge Institute for Medical Research, University of Cambridge, Addenbrooke's Hospital, Cambridge CB2 0XY, UK, Department of Pathology, Xiangya School of Medicine, Central South University, Changsha 410008, Hunan, P.R. China, Department of Clinic Test, Xiangya Hospital, Central South University, Changsha 410008, Hunan, P.R. China, Department of Geriatric Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, P.R. China
Published online on: April 20, 2012 https://doi.org/10.3892/or.2012.1769
Pages: 77-84

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Abstract

Disturbance of miRNA expression may play a key role in the initiation and progression of colorectal cancer (CRC). CRC should be viewed as a heterogeneous disease, but previous studies have only screened dysregulated miRNAs in CRC from a panel of 96, 145, 287 and 455 miRNAs, respectively. It is necessary to identify new aberrantly expressed miRNAs in rectal cancer. In this study tissue samples were derived from patients undergoing a surgical procedure to remove a portion of cancers. The expression profile of 904 miRNAs was analyzed using a miRCURY™ LNA Array from 6-paired rectal cancers and normal tissues. The expression levels of 4 miRNAs were compared by real-time PCR between colon and rectal cancer, and also the expression levels of metastatic miRNAs in different stages of rectal cancer were analyzed. We found that 67 miRNA precursors are upregulated in rectal cancer (p<0.05) and 21 of those have never been reported in colorectal cancer (CRC); 39 miRNA precursors are downregulated (p<0.05) and 24 novel dysregulated miRNAs were identified in rectal cancer. miR-31, miR-126 and miR-143 are differentially expressed between colon cancer and rectal cancer. Here, we report an miRNA profile of rectal cancer, and we identified differential expression patterns of miRNAs between rectal and colon cancers. This novel information may suggest the potential roles of these miRNAs in the diagnosis of rectal cancer.