Open Access

Replicating retroviral vectors for oncolytic virotherapy of experimental hepatocellular carcinoma

  • Authors:
    • Yin-Che Lu
    • Ying-Ju Chen
    • Yuan-Ren Yu
    • Yi-Hui Lai
    • Jen-Chieh Cheng
    • Yu-Fen Li
    • Cheng-Huang Shen
    • Chien-Kuo Tai
  • View Affiliations

  • Published online on: April 26, 2012     https://doi.org/10.3892/or.2012.1789
  • Pages: 21-26
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Abstract

Gene therapy mediated by murine leukemia virus (MLV)-based replicating retrovirus vector (RRV) was previously proven to be highly effective in tumor cell killing, resulting in significant suppression of tumor growth in vivo. Recently, we developed a different form of RRV which is derived from another retrovirus, gibbon ape leukemia virus (GALV), as a cancer therapeutic agent. We compared the gene delivery efficiency and antitumor effects in the two types of RRV in experimental hepatocellular carcinoma (HCC). Our results show that both RRVs can efficiently spread throughout entire HCC cell populations in vitro and achieve high transduction efficiency in HCC xenografts in vivo, while GALV RRV, in general, exhibited more rapid replication kinetics in the tumors. In vitro, substantial HCC cell killing was achieved even when initially only 1% of the HCC cells were producing RRVs that express the yeast cytosine deaminase suicide gene, indicating that the high efficiency of gene transfer by replicative spread of RRVs greatly increased suicide gene toxicity. In vivo, GALV RRV-mediated suicide gene therapy efficiently suppressed HCC tumor growth and no detectable RRV signals were observed in extratumoral tissues, showing promise in using GALV RRV as a cancer therapeutic agent.

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Journal Cover

July 2012
Volume 28 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lu Y, Chen Y, Yu Y, Lai Y, Cheng J, Li Y, Shen C and Tai C: Replicating retroviral vectors for oncolytic virotherapy of experimental hepatocellular carcinoma. Oncol Rep 28: 21-26, 2012
APA
Lu, Y., Chen, Y., Yu, Y., Lai, Y., Cheng, J., Li, Y. ... Tai, C. (2012). Replicating retroviral vectors for oncolytic virotherapy of experimental hepatocellular carcinoma. Oncology Reports, 28, 21-26. https://doi.org/10.3892/or.2012.1789
MLA
Lu, Y., Chen, Y., Yu, Y., Lai, Y., Cheng, J., Li, Y., Shen, C., Tai, C."Replicating retroviral vectors for oncolytic virotherapy of experimental hepatocellular carcinoma". Oncology Reports 28.1 (2012): 21-26.
Chicago
Lu, Y., Chen, Y., Yu, Y., Lai, Y., Cheng, J., Li, Y., Shen, C., Tai, C."Replicating retroviral vectors for oncolytic virotherapy of experimental hepatocellular carcinoma". Oncology Reports 28, no. 1 (2012): 21-26. https://doi.org/10.3892/or.2012.1789