Open Access

Small breast epithelial mucin promotes the invasion and metastasis of breast cancer cells via promoting epithelial‑to‑mesenchymal transition

  • Authors:
    • Qiu‑Hua Li
    • Zhao‑Zhe Liu
    • Ya‑Νan Ge
    • Xing Liu
    • Xiao‑Dong Xie
    • Zhen‑Dong Zheng
    • Yue‑Hai Ma
    • Bin Liu
  • View Affiliations

  • Published online on: June 9, 2020     https://doi.org/10.3892/or.2020.7640
  • Pages: 509-518
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to observe the influence of the small breast epithelial mucin (MUCL1) (also known as SBEM) gene on migration and invasion ability of breast cancer cells and to explore the potentially involved mechanism. SBEM‑interference plasmid and SBEM‑overexpressing plasmid were constructed. SBEM‑knockdown or SBEM‑​overexpressing MCF‑7 and MDA‑MB‑231 breast cancer cells were established by lentivirus‑mediated stable transfection method. The scratch wound‑healing assay and Transwell chamber experiment were used to detect the influence of the SBEM gene on the migration and invasion abilities of MCF‑7 and MDA‑MB‑231 cells. Real‑time PCR (polymerase chain reaction) and western blotting were used to detect the expression of epithelial‑to‑mesenchymal transition (EMT)‑related markers and regulators. The cell morphology was observed after transfection. The SBEM‑knockdown or SBEM‑overexpressing MCF‑7 and MDA‑MB‑231 cells were established successfully. The migration and invasion abilities were decreased after SBEM was downregulated, and were increased after SBEM was overexpressed both in MCF‑7 and MDA‑MB‑231 cell lines. The mRNA and protein expressions of N‑cadherin, Twist and vimentin were elevated following SBEM overexpression, while the expression of E‑cadherin and claudin‑1 were found to be decreased following SBEM overexpression. In conclusion, SBEM has the potential to promote migration and invasion ability of breast cancer cells via promoting epithelial‑to‑mesenchymal transition.

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August-2020
Volume 44 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Li, Q., Liu, Z., Ge, Y., Liu, X., Xie, X., Zheng, Z. ... Liu, B. (2020). Small breast epithelial mucin promotes the invasion and metastasis of breast cancer cells via promoting epithelial‑to‑mesenchymal transition. Oncology Reports, 44, 509-518. https://doi.org/10.3892/or.2020.7640
MLA
Li, Q., Liu, Z., Ge, Y., Liu, X., Xie, X., Zheng, Z., Ma, Y., Liu, B."Small breast epithelial mucin promotes the invasion and metastasis of breast cancer cells via promoting epithelial‑to‑mesenchymal transition". Oncology Reports 44.2 (2020): 509-518.
Chicago
Li, Q., Liu, Z., Ge, Y., Liu, X., Xie, X., Zheng, Z., Ma, Y., Liu, B."Small breast epithelial mucin promotes the invasion and metastasis of breast cancer cells via promoting epithelial‑to‑mesenchymal transition". Oncology Reports 44, no. 2 (2020): 509-518. https://doi.org/10.3892/or.2020.7640