GTP-binding protein and its associated event in membranes from endometrial carcinoma

  • Authors:
    • H Takagi
    • A Imai
    • S Horibe
    • T Fuseya
    • T Tamaya
  • View Affiliations

  • Published online on: January 1, 1996     https://doi.org/10.3892/or.3.1.161
  • Pages: 161-163
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Abstract

Endometrial carcinoma expresses gonadotropin-releasing hormone receptor (Gn-RHR) known to be linked by heterotrimeric GTP-binding proteins (G proteins). We have examined endometrial carcinomas for the presence of G protein and its associated membrane event in isolated plasma membranes. Endometrial carcinomas surgically removed had been screened for Gn-RHR expression prior to plasma membrane isolation. G protein level was observed by immunoblotting of membrane extracts with anti-G protein antibodies. G protein-associated membrane event was observed by phosphotyrosine phosphatase (PTP) activity determined with the synthetic substrate p-nitrophenyl in a spectrophotometric assay. Immunoblotting with the specific G alpha 11 and G alpha s antibodies showed significant levels of 42-kDa and 46-kDa proteins in endometrial carcinoma membranes, respectively. The nonhydrolyzable GTP analog GTP-gamma-S stimulated the phosphatase activity in a dose-dependent manner: a half maximal effect occurred with 30 nM. These findings demonstrate the expression of G proteins and the presence G proteins-associated membrane event in endometrial carcinoma.

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January 1996
Volume 3 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Takagi H, Imai A, Horibe S, Fuseya T and Tamaya T: GTP-binding protein and its associated event in membranes from endometrial carcinoma. Oncol Rep 3: 161-163, 1996.
APA
Takagi, H., Imai, A., Horibe, S., Fuseya, T., & Tamaya, T. (1996). GTP-binding protein and its associated event in membranes from endometrial carcinoma. Oncology Reports, 3, 161-163. https://doi.org/10.3892/or.3.1.161
MLA
Takagi, H., Imai, A., Horibe, S., Fuseya, T., Tamaya, T."GTP-binding protein and its associated event in membranes from endometrial carcinoma". Oncology Reports 3.1 (1996): 161-163.
Chicago
Takagi, H., Imai, A., Horibe, S., Fuseya, T., Tamaya, T."GTP-binding protein and its associated event in membranes from endometrial carcinoma". Oncology Reports 3, no. 1 (1996): 161-163. https://doi.org/10.3892/or.3.1.161