p53-expression and chromosome 17-copy number in colorectal adenomas

  • Authors:
    • G Baretton
    • M Vogt
    • J Diebold
    • S Blasenbreu
    • K Schneiderbanger
    • U Lohrs
  • View Affiliations

  • Published online on: March 1, 1996     https://doi.org/10.3892/or.3.2.357
  • Pages: 357-363
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Abstract

To get better insight into the role of numerical chromosome 17-aberrations and functional loss of the tumor suppressor gene TP53 during the early steps of colorectal carcinogenesis we analyzed paraffin-embedded tumor tissue from 58 colorectal adenomas with different histological features for p53-expression by immunohistology (IH; moAb DO1), #17-copy number by interphase-cytogenetics [nonradioactive in situ-hybridization (NISH) with a centromer-specific DNA-probe (D17Z1)], and DNA-ploidy by flow cytometry (FCM) with special emphasis on histopathological correlation. Seven adenomas (12%) showed nuclear p53-immunoreactivity. p53-expression was correlated with villous/tubulo-villous growth pattern (p=0.005) and grade of atypia (p=0.003) or dysplasia (p=0.0018). #17-aneusomy was present in 31% of the cases (29% deletions, 2% gains). In the FCM-analysis, 33% of the adenomas were DNA-non-diploid. p53-immunoreactivity correlated statistically significantly with FCM-non-diploidy (p=0.004) suggesting a role for the TP53-gene in the aneuploidization process. #17-deletions were associated to p53-immunoreactivity (p=0.046) but no correlation was found between FCM-ploidy and #17-copy number. As for loss of a tumor suppressor gene both alleles have to be affected, our data suggest a multistep process of TP53-inactivation. Whether the subgroup of adenomas with p53-expression might progress faster into invasive carcinoma than p53-negative cases remains to be tested.

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March 1996
Volume 3 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Baretton G, Vogt M, Diebold J, Blasenbreu S, Schneiderbanger K and Lohrs U: p53-expression and chromosome 17-copy number in colorectal adenomas. Oncol Rep 3: 357-363, 1996.
APA
Baretton, G., Vogt, M., Diebold, J., Blasenbreu, S., Schneiderbanger, K., & Lohrs, U. (1996). p53-expression and chromosome 17-copy number in colorectal adenomas. Oncology Reports, 3, 357-363. https://doi.org/10.3892/or.3.2.357
MLA
Baretton, G., Vogt, M., Diebold, J., Blasenbreu, S., Schneiderbanger, K., Lohrs, U."p53-expression and chromosome 17-copy number in colorectal adenomas". Oncology Reports 3.2 (1996): 357-363.
Chicago
Baretton, G., Vogt, M., Diebold, J., Blasenbreu, S., Schneiderbanger, K., Lohrs, U."p53-expression and chromosome 17-copy number in colorectal adenomas". Oncology Reports 3, no. 2 (1996): 357-363. https://doi.org/10.3892/or.3.2.357