DNA-protein crosslinks as a biomarker of cis-platinum activity in cancer patients

  • Authors:
    • E Taioli
    • A Zhitkovich
    • P Toniolo
    • J Bernstein
    • R Blum
    • M Costa
  • View Affiliations

  • Published online on: May 1, 1996     https://doi.org/10.3892/or.3.3.439
  • Pages: 439-441
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Abstract

We have developed a new method to assess the amount of DNA-protein crosslinks (DNA-PC) in peripheral lymphocytes, based on the selective precipitation of the DNA crosslinked to proteins. We assessed the amount of DNA-PC in peripheral lymphocytes of 18 cancer patients who underwent chemotherapy with cis-platinum for the first time. Since the chemotherapy was administered over a two-day period, blood samples were drawn at baseline (before starting the therapy), 4 h after the infusion of the first dose of cis-platinum, the next day (24 h after the first dose, and immediately before the infusion of the second dose), and 2 days later (48 h after the first dose). The mean change of DNA-PC 4 h after therapy was 0.8+/-0.8% (p=0.006), 0.5+/-0.6% after 1 day (p=0.007), and 0.1+/-0.5% after two days (ns). The correlation between DNA-PC changes and cumulative dose of cis-platinum was -0.22 at 4 h, -0.19 after 1 day, and -0.68 at 2 days (p=0.005). The crosslinking effect of cis-platinum seems to vary among individuals and with dose; the DNA-PC may be used to define sub-populations of patients with various degree of sensitivity to the pharmacologic action of this chemotherapeutic agent, and thus to adjust the dosage on an individual basis.

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May 1996
Volume 3 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Taioli E, Zhitkovich A, Toniolo P, Bernstein J, Blum R and Costa M: DNA-protein crosslinks as a biomarker of cis-platinum activity in cancer patients. Oncol Rep 3: 439-441, 1996.
APA
Taioli, E., Zhitkovich, A., Toniolo, P., Bernstein, J., Blum, R., & Costa, M. (1996). DNA-protein crosslinks as a biomarker of cis-platinum activity in cancer patients. Oncology Reports, 3, 439-441. https://doi.org/10.3892/or.3.3.439
MLA
Taioli, E., Zhitkovich, A., Toniolo, P., Bernstein, J., Blum, R., Costa, M."DNA-protein crosslinks as a biomarker of cis-platinum activity in cancer patients". Oncology Reports 3.3 (1996): 439-441.
Chicago
Taioli, E., Zhitkovich, A., Toniolo, P., Bernstein, J., Blum, R., Costa, M."DNA-protein crosslinks as a biomarker of cis-platinum activity in cancer patients". Oncology Reports 3, no. 3 (1996): 439-441. https://doi.org/10.3892/or.3.3.439