Thymidine phosphorylase activity required for tumor angiogenesis and growth

  • Authors:
    • K Nishimoto
    • K Miyadera
    • Y Takebayashi
    • K Fukuda
    • M Haraguchi
    • T Furukawa
    • Y Yamada
    • S Akiyama
  • View Affiliations

  • Published online on: January 1, 1997     https://doi.org/10.3892/or.4.1.55
  • Pages: 55-58
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Abstract

Thymidine phosphorylase (TP) is identical to platelet-derived endothelial cell growth factor (PD-ECGF), and has angiogenic activity. We examined the involvement of TP activity in tumor growth and angiogenesis. KB cells were transfected with wild-type or mutant (L148R) PD-ECGF cDNA, and two sublines with high TP activity, KB/wt4 and KB/wt6, and one subline with no TP activity, KB/L148R, were cloned, respectively. The doubling times of these subclones in vitro were similar to that of KB cells. However, the growth of KB/wt4 and KB/wt6 cells was significantly faster when xenografted into nude mice than that of control cells with no TP activity. The tumors with high TP activity (KB/wt4 and KB/wt6) had significantly more microvessels than those with no TP activity (KB/-, KB/CV and KB/L148R) (P<0.01). These results, taken together with previous reports, suggest that the TP enzyme activity itself is involved in angiogenesis and growth of the KB tumors.

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January 1997
Volume 4 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Nishimoto K, Miyadera K, Takebayashi Y, Fukuda K, Haraguchi M, Furukawa T, Yamada Y and Akiyama S: Thymidine phosphorylase activity required for tumor angiogenesis and growth. Oncol Rep 4: 55-58, 1997
APA
Nishimoto, K., Miyadera, K., Takebayashi, Y., Fukuda, K., Haraguchi, M., Furukawa, T. ... Akiyama, S. (1997). Thymidine phosphorylase activity required for tumor angiogenesis and growth. Oncology Reports, 4, 55-58. https://doi.org/10.3892/or.4.1.55
MLA
Nishimoto, K., Miyadera, K., Takebayashi, Y., Fukuda, K., Haraguchi, M., Furukawa, T., Yamada, Y., Akiyama, S."Thymidine phosphorylase activity required for tumor angiogenesis and growth". Oncology Reports 4.1 (1997): 55-58.
Chicago
Nishimoto, K., Miyadera, K., Takebayashi, Y., Fukuda, K., Haraguchi, M., Furukawa, T., Yamada, Y., Akiyama, S."Thymidine phosphorylase activity required for tumor angiogenesis and growth". Oncology Reports 4, no. 1 (1997): 55-58. https://doi.org/10.3892/or.4.1.55