Combination therapy with podophyllin and vidarabine for human papillomavirus positive cervical intraepithelial neoplasia.

  • Authors:
    • A Okamoto
    • C D Woodworth
    • K Yen
    • J Chung
    • S Isonishi
    • T Nikaido
    • T Kiyokawa
    • H Seo
    • Y Kitahara
    • K Ochiai
    • T Tanaka
  • View Affiliations

  • Published online on: March 1, 1999     https://doi.org/10.3892/or.6.2.269
  • Pages: 269-345
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Abstract

Our aim was to establish an effective non-surgical treatment for cervical intraepithelial neoplasia (CIN) through inactivation of human papillomavirus (HPV), the major etiological agent for this disease. We show that vidarabine, a DNA polymerase inhibitor, suppressed growth and HPV gene expression in human cervical keratinocytes immortalized by HPV or in cervical cancer cell lines. Expression of HPV-16 E6 and E7 proteins in normal cervical keratinocytes sensitized cells to apoptosis in the presence of podophyllin or vidarabine. We applied vidarabine ointment and/or podophyllin to cervical epithelium in 28 cases of CIN I-II to evaluate the therapeutic effectiveness of these agents. Co-application of vidarabine and podophyllin in six treatments caused regression of lesions cytologically and histologically, and disappearance of HPV-16 or -18 DNA in 17 of 21 (81%) women. Our results suggest that the combination of vidarabine and podophyllin therapy is an effective non-surgical treatment for HPV-positive CIN.

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Mar-Apr 1999
Volume 6 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Okamoto A, Woodworth C, Yen K, Chung J, Isonishi S, Nikaido T, Kiyokawa T, Seo H, Kitahara Y, Ochiai K, Ochiai K, et al: Combination therapy with podophyllin and vidarabine for human papillomavirus positive cervical intraepithelial neoplasia.. Oncol Rep 6: 269-345, 1999
APA
Okamoto, A., Woodworth, C., Yen, K., Chung, J., Isonishi, S., Nikaido, T. ... Tanaka, T. (1999). Combination therapy with podophyllin and vidarabine for human papillomavirus positive cervical intraepithelial neoplasia.. Oncology Reports, 6, 269-345. https://doi.org/10.3892/or.6.2.269
MLA
Okamoto, A., Woodworth, C., Yen, K., Chung, J., Isonishi, S., Nikaido, T., Kiyokawa, T., Seo, H., Kitahara, Y., Ochiai, K., Tanaka, T."Combination therapy with podophyllin and vidarabine for human papillomavirus positive cervical intraepithelial neoplasia.". Oncology Reports 6.2 (1999): 269-345.
Chicago
Okamoto, A., Woodworth, C., Yen, K., Chung, J., Isonishi, S., Nikaido, T., Kiyokawa, T., Seo, H., Kitahara, Y., Ochiai, K., Tanaka, T."Combination therapy with podophyllin and vidarabine for human papillomavirus positive cervical intraepithelial neoplasia.". Oncology Reports 6, no. 2 (1999): 269-345. https://doi.org/10.3892/or.6.2.269