Antagonistic effect of EGF against TGF beta1 on transformed phenotype and tropomyosin expression of human lung carcinoma A549 cells.
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- Published online on: November 1, 2000 https://doi.org/10.3892/or.7.6.1323
- Pages: 1323-1329
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Abstract
TGF beta1 suppressed the colony forming ability of human lung carcinoma cell line, A549 cells in a dose-dependent manner. Accompanying these changes, the levels of the expression of high-molecular weight tropomyosin (TM) isoforms, especially TM1 and TM2, were significantly increased in the TGF beta1-treated A549 cells in a dose-dependent manner. When A549 cells were treated with TGF beta1 (2.5 pM) and EGF (1 nM) simultaneously, anchorage-independent growth of A549 cells was restored. In these cells, the expression of high-molecular weight TM decreased. The results suggest that the levels of the expression of high-molecular weight TM may be involved in antagonistic effects of EGF and TGF beta1 on the anchorage-independent growth of A549 cells.
