Leucine zipper structure of TSC-22 (TGF-β stimulated clone-22) markedly inhibits the anchorage-independent growth of salivary gland cancer cells

  • Authors:
    • S. Hino
    • H. Kawamata
    • F. Omotehara
    • D. Uchida
    • N.-M. Begum
    • H. Yoshida
    • M. Sato
    • T. Fujimori
  • View Affiliations

  • Published online on: March 1, 2002     https://doi.org/10.3892/or.9.2.371
  • Pages: 371-374
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Abstract

Several investigators have demonstrated that TGF-β stimulated clone-22 (TSC-22) regulates cell growth and differentiation, and cell death. TSC-22 is a putative transcriptional regulator containing a leucine zipper-like structure and a nuclear export signal. We previously showed the cytoplasmic localization of TSC-22 and the nuclear translocation of TSC-22 concomitant with induction of apoptosis in salivary gland cancer cells. In the present study, we attempted to identify the active domain of TSC-22 protein that regulated the biological functions of TSC-22. We constructed three mammalian expression vectors, which could produce full length TSC-22 only in cytoplasm, the leucine zipper structure of TSC-22 in both cytoplasm and nucleus, and the leucine zipper structure only in nucleus. Then, we transfected a salivary gland cancer cell line, HSG with these expression vectors, and investigated the growth profile of the transfectants. None of the TSC-22 constructs inhibited the monolayer growth and the anchorage-dependent colony formation of HSG cells. However, the leucine zipper structure of TSC-22 markedly inhibited the anchorage-independent colony formation of HSG cells (p<0.001; one way ANOVA). Full length TSC-22 also suppressed the anchorage-independent colony formation of HSG cells, although the effect of full length TSC-22 was much lower than those of the leucine zipper constructs. These observations suggest that the leucine zipper structure in TSC-22 protein is an active domain that negatively regulates the growth of salivary gland cancer cells.

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March 2002
Volume 9 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Hino, S., Kawamata, H., Omotehara, F., Uchida, D., Begum, N., Yoshida, H. ... Fujimori, T. (2002). Leucine zipper structure of TSC-22 (TGF-β stimulated clone-22) markedly inhibits the anchorage-independent growth of salivary gland cancer cells. Oncology Reports, 9, 371-374. https://doi.org/10.3892/or.9.2.371
MLA
Hino, S., Kawamata, H., Omotehara, F., Uchida, D., Begum, N., Yoshida, H., Sato, M., Fujimori, T."Leucine zipper structure of TSC-22 (TGF-β stimulated clone-22) markedly inhibits the anchorage-independent growth of salivary gland cancer cells". Oncology Reports 9.2 (2002): 371-374.
Chicago
Hino, S., Kawamata, H., Omotehara, F., Uchida, D., Begum, N., Yoshida, H., Sato, M., Fujimori, T."Leucine zipper structure of TSC-22 (TGF-β stimulated clone-22) markedly inhibits the anchorage-independent growth of salivary gland cancer cells". Oncology Reports 9, no. 2 (2002): 371-374. https://doi.org/10.3892/or.9.2.371