Successive cultures of mature hepatocytes for hepatocyte autotransplantation to assist liver function after liver resection for cancer
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- Published online on: July 1, 2002 https://doi.org/10.3892/or.9.4.713
- Pages: 713-721
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Abstract
We developed a method for the rapid successive cultures of adult rat mature hepatocytes on plastic dishes while avoiding viral transformation or co-culture with other cell lines. This method also allows for culturing adult human mature hepatocytes up to the secondary culture. These can be expected to provide a good source for hepatocyte autotransplantation, and, combined with the previously reported methods for the transplantation of hepatocytes into the spleen, a promising option for the support of liver function after liver resection for cancer without the need for immunosuppressive agents.
