Analysis of cyclin D1 and CDK expression in colonic polyps containing neoplastic foci: A study of proteins extracted from paraffin sections

Izawa,Hikaru; Yamamoto,Hirofumi; Ikeda,Masataka; Ikeda,Kimimasa; Fukunaga,Hiroki; Yasui,Masayoshi; Ikenaga,Masakazu; Sekimoto,Mitsugu; Monden,Takushi; Matsuura,Nariaki; Monden,Morito

doi:10.3892/or.9.6.1313

November-December 2002 Volume 9 Issue 6

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November-December 2002 Volume 9 Issue 6

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Article

Analysis of cyclin D1 and CDK expression in colonic polyps containing neoplastic foci: A study of proteins extracted from paraffin sections

Authors:
- Hikaru Izawa
- Hirofumi Yamamoto
- Masataka Ikeda
- Kimimasa Ikeda
- Hiroki Fukunaga
- Masayoshi Yasui
- Masakazu Ikenaga
- Mitsugu Sekimoto
- Takushi Monden
- Nariaki Matsuura
- Morito Monden
View Affiliations / Copyright

Affiliations: Department of Surgery and Clinical Oncology, Graduate School of Medicine, Osaka University, Osaka, Japan
Pages: 1313-1318
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Published online on: November 1, 2002

https://doi.org/10.3892/or.9.6.1313
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Abstract

Overexpression or amplification of G1 cyclins has been demonstrated in various types of human cancers. Overexpression of cyclin D1, an accelerator of cell cycle, is thought to be an early event in colonic multistage carcinogenesis, but its expression at transformation from benign to neoplastic foci is not clear. We analyzed the expression of cyclin D1 and its catalystic partner CDK4 in colon polyps containing neoplastic foci. For direct comparison of the expression in adenomatous tissues and neoplastic foci, proteins in adequate amounts were extracted from paraffin embedded sections. Western blot and densitometry analyses showed that cyclin D1 expression was 2.3-times and 2.5-times higher in adenomatous tissues and neoplastic foci, compared with normal colonic mucosa. In contrast, the levels of CDK4 and CDK2 expression were only modestly increased in adenomatous tissues but significantly higher in neoplastic foci, relative to normal mucosa. Expression of PCNA, a cell proliferation marker, increased from normal mucosa to adenoma to focal cancer. Our findings suggest that overexpression of cyclin D1 may be associated with high proliferative activity in adenomatous tissues and that concurrent high expression of cyclin D1 and CDK4 may further perturb cell cycle progression and play a pivotal role in colonic carcinogenesis.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Analysis of cyclin D1 and CDK expression in colonic polyps containing neoplastic foci: A study of proteins extracted from paraffin sections

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Abstract

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