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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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February 2009 Volume 21 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

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Article

Establishment of a lymph node metastasis model from subcutaneous tumors of gastrointestinal stromal tumor model cells

  • Authors:
    • Kazufumi Sakurama
    • Yoshio Naomoto
    • Toshiaki Ohara
    • Nobuyuki Watanabe
    • Munenori Takaoka
    • Hitoshi Nagatsuka
    • Yasuko Tomono
    • Toru Tanida
    • Kazuhiro Noma
    • Shunsuke Tanabe
    • Yasuhiro Fujiwara
    • Takayuki Motoki
    • Yasuhiro Shirakawa
    • Tomoki Yamatsuji
    • Seiichi Hirota
    • Takahiro Taguchi
    • Noriaki Tanaka
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterological Surgery, Transplant and Surgical Oncology, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan
  • Pages: 407-411
    |
    Published online on: February 1, 2009
       https://doi.org/10.3892/or_00000237
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Abstract

There is currently no suitable animal model of metastasis using cultured human gastrointestinal stromal tumor cells even though the molecular mechanisms of c-KIT-mediated progression and metastasis should be clarified. Ba/F3 murine lymphocyte cells transduced with mutant c-KIT have been utilized to analyze some molecular mechanisms related to a constitutively activated c-KIT signaling and to assess the efficacy of molecular-targeted inhibitors. Using this cellular system, we coincidentally discovered the development of axillary and inguinal lymph node swelling three weeks after subcutaneous injection of Ba/F3 cells with c-KIT mutation into nude mice. Mutation-specific PCR detected c-KIT mutation in the swollen lymph nodes but not in unmetastasized normal lymph nodes, indicating that the lymph nodes contain tumor cells which should come from a primary subcutaneous tumor. Microscopic observation revealed tumor cells infiltrating through lymphatic follicles with Ki-67-positive staining to distinguish them from lymphocytes. The significance of this model is helpful to understand the molecular mechanisms of c-KIT-mediated metastasis and is useful for assessments of molecular therapeutics and in vivo imaging.

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Copy and paste a formatted citation
Spandidos Publications style
Sakurama K, Naomoto Y, Ohara T, Watanabe N, Takaoka M, Nagatsuka H, Tomono Y, Tanida T, Noma K, Tanabe S, Tanabe S, et al: Establishment of a lymph node metastasis model from subcutaneous tumors of gastrointestinal stromal tumor model cells. Oncol Rep 21: 407-411, 2009.
APA
Sakurama, K., Naomoto, Y., Ohara, T., Watanabe, N., Takaoka, M., Nagatsuka, H. ... Tanaka, N. (2009). Establishment of a lymph node metastasis model from subcutaneous tumors of gastrointestinal stromal tumor model cells. Oncology Reports, 21, 407-411. https://doi.org/10.3892/or_00000237
MLA
Sakurama, K., Naomoto, Y., Ohara, T., Watanabe, N., Takaoka, M., Nagatsuka, H., Tomono, Y., Tanida, T., Noma, K., Tanabe, S., Fujiwara, Y., Motoki, T., Shirakawa, Y., Yamatsuji, T., Hirota, S., Taguchi, T., Tanaka, N."Establishment of a lymph node metastasis model from subcutaneous tumors of gastrointestinal stromal tumor model cells". Oncology Reports 21.2 (2009): 407-411.
Chicago
Sakurama, K., Naomoto, Y., Ohara, T., Watanabe, N., Takaoka, M., Nagatsuka, H., Tomono, Y., Tanida, T., Noma, K., Tanabe, S., Fujiwara, Y., Motoki, T., Shirakawa, Y., Yamatsuji, T., Hirota, S., Taguchi, T., Tanaka, N."Establishment of a lymph node metastasis model from subcutaneous tumors of gastrointestinal stromal tumor model cells". Oncology Reports 21, no. 2 (2009): 407-411. https://doi.org/10.3892/or_00000237
Copy and paste a formatted citation
x
Spandidos Publications style
Sakurama K, Naomoto Y, Ohara T, Watanabe N, Takaoka M, Nagatsuka H, Tomono Y, Tanida T, Noma K, Tanabe S, Tanabe S, et al: Establishment of a lymph node metastasis model from subcutaneous tumors of gastrointestinal stromal tumor model cells. Oncol Rep 21: 407-411, 2009.
APA
Sakurama, K., Naomoto, Y., Ohara, T., Watanabe, N., Takaoka, M., Nagatsuka, H. ... Tanaka, N. (2009). Establishment of a lymph node metastasis model from subcutaneous tumors of gastrointestinal stromal tumor model cells. Oncology Reports, 21, 407-411. https://doi.org/10.3892/or_00000237
MLA
Sakurama, K., Naomoto, Y., Ohara, T., Watanabe, N., Takaoka, M., Nagatsuka, H., Tomono, Y., Tanida, T., Noma, K., Tanabe, S., Fujiwara, Y., Motoki, T., Shirakawa, Y., Yamatsuji, T., Hirota, S., Taguchi, T., Tanaka, N."Establishment of a lymph node metastasis model from subcutaneous tumors of gastrointestinal stromal tumor model cells". Oncology Reports 21.2 (2009): 407-411.
Chicago
Sakurama, K., Naomoto, Y., Ohara, T., Watanabe, N., Takaoka, M., Nagatsuka, H., Tomono, Y., Tanida, T., Noma, K., Tanabe, S., Fujiwara, Y., Motoki, T., Shirakawa, Y., Yamatsuji, T., Hirota, S., Taguchi, T., Tanaka, N."Establishment of a lymph node metastasis model from subcutaneous tumors of gastrointestinal stromal tumor model cells". Oncology Reports 21, no. 2 (2009): 407-411. https://doi.org/10.3892/or_00000237
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