Cross-resistance of platinum derivatives in H-1R, a cisplatin-resistant cell line

  • Authors:
    • Kenji Negoro
    • Yukio Yamano
    • Dai Nakashima
    • Kengo Saito
    • Ken Nakatani
    • Masashi Shiiba
    • Hiroki Bukawa
    • Hidetaka Yokoe
    • Katsuhiro Uzawa
    • Takeshi Wada
    • Hideki Tanzawa
    • Shigeyuki Fujita
  • View Affiliations

  • Published online on: February 1, 2009     https://doi.org/10.3892/or_00000243
  • Pages: 443-449
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Abstract

We previously established H-1R cells, a cisplatin (CDDP)-resistant cell line, from H-1 cells, a CDDP-sensitive oral carcinoma cell line. The aim of this study was to identify the molecular mechanism of cross-resistance to antitumor drugs containing a platinum agent in H-1R cells. The 3-(3,4-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay and clonogenecity assay indicated that H-1R cells showed strong cross-resistance to carboplatin, nedaplatin and oxaliplatin. The expression status of the copper transporter and organic cation transporters was confirmed by real-time quantitative reverse transcriptase-polymerase chain reaction. The transporters ATP7A, ATP7B, hCtr1, hOCT1 and hOCT2 were up-regulated, whereas hOCT3 was down-regulated. The cellular glutathione level was elevated 2-fold in H-1R cells compared with H-1 cells. Our results suggested that H-1 and H-1R cells may be useful in searching for candidate genes responsible for cross-resistance to platinum derivatives and for further studies to understand the mechanism of platinum resistance.

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February 2009
Volume 21 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Negoro K, Yamano Y, Nakashima D, Saito K, Nakatani K, Shiiba M, Bukawa H, Yokoe H, Uzawa K, Wada T, Wada T, et al: Cross-resistance of platinum derivatives in H-1R, a cisplatin-resistant cell line. Oncol Rep 21: 443-449, 2009.
APA
Negoro, K., Yamano, Y., Nakashima, D., Saito, K., Nakatani, K., Shiiba, M. ... Fujita, S. (2009). Cross-resistance of platinum derivatives in H-1R, a cisplatin-resistant cell line. Oncology Reports, 21, 443-449. https://doi.org/10.3892/or_00000243
MLA
Negoro, K., Yamano, Y., Nakashima, D., Saito, K., Nakatani, K., Shiiba, M., Bukawa, H., Yokoe, H., Uzawa, K., Wada, T., Tanzawa, H., Fujita, S."Cross-resistance of platinum derivatives in H-1R, a cisplatin-resistant cell line". Oncology Reports 21.2 (2009): 443-449.
Chicago
Negoro, K., Yamano, Y., Nakashima, D., Saito, K., Nakatani, K., Shiiba, M., Bukawa, H., Yokoe, H., Uzawa, K., Wada, T., Tanzawa, H., Fujita, S."Cross-resistance of platinum derivatives in H-1R, a cisplatin-resistant cell line". Oncology Reports 21, no. 2 (2009): 443-449. https://doi.org/10.3892/or_00000243