Cytotoxicity and radiosensitization effect of TRA-8 on radioresistant human larynx squamous carcinoma cells

  • Authors:
    • F. Wu
    • Y. Hu
    • J. Long
    • Y. J. Zhou
    • Y. H. Zhong
    • Z. K. Liao
    • S. Q. Liu
    • F. X. Zhou
    • C. H. Xie
  • View Affiliations

  • Published online on: February 1, 2009     https://doi.org/10.3892/or_00000245
  • Pages: 461-465
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Abstract

TRAIL induces apoptosis in a variety of tumorigenic and transformed cell lines, but not in many normal cells. Recent studies have demonstrated that death receptor 5 (DR5), one of the two death receptors bound by TRAIL, showed expression in most malignantly transformed cells. This study evaluated effects of a monoclonal antibody (TRA-8) to human death receptor 5, combined with ionizing radiation, on radioresistant human larynx squamous carcinoma cell line (Hep-2R). Cells were treated with TRA-8 alone or in combination with radiation, cell viability inhibition was measured by MTT assay, and the induction of apoptosis was determined by Annexin V staining. Radionsensitivity of Hep-2R cells treated with TRA-8 were investigated with long-term clonogenic assays. Regulation of DR5 expression in cells after radiation was analyzed by indirect immunofluorescence using murine TRA-8 in combination with flow cytometry. The results suggested that TRA-8 enhanced radionsensitivity of Hep-2R cells, and that TRA-8 regulated Hep-2R cell cycle arrest at G2/M phase. Irradiation up-regulated the expression of DR5, and when combined with TRA-8 yielded optimal survival benefit. Therefore, TRA-8 can be used in combination with irradiation in radioresistant human larynx squamous carcinoma cells. Monoclonal antibodies such as TRA-8 may play an important role in the development of an effective treatment strategy for patients with radioresistant cancers.

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February 2009
Volume 21 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wu F, Hu Y, Long J, Zhou YJ, Zhong YH, Liao ZK, Liu SQ, Zhou FX and Xie CH: Cytotoxicity and radiosensitization effect of TRA-8 on radioresistant human larynx squamous carcinoma cells. Oncol Rep 21: 461-465, 2009
APA
Wu, F., Hu, Y., Long, J., Zhou, Y.J., Zhong, Y.H., Liao, Z.K. ... Xie, C.H. (2009). Cytotoxicity and radiosensitization effect of TRA-8 on radioresistant human larynx squamous carcinoma cells. Oncology Reports, 21, 461-465. https://doi.org/10.3892/or_00000245
MLA
Wu, F., Hu, Y., Long, J., Zhou, Y. J., Zhong, Y. H., Liao, Z. K., Liu, S. Q., Zhou, F. X., Xie, C. H."Cytotoxicity and radiosensitization effect of TRA-8 on radioresistant human larynx squamous carcinoma cells". Oncology Reports 21.2 (2009): 461-465.
Chicago
Wu, F., Hu, Y., Long, J., Zhou, Y. J., Zhong, Y. H., Liao, Z. K., Liu, S. Q., Zhou, F. X., Xie, C. H."Cytotoxicity and radiosensitization effect of TRA-8 on radioresistant human larynx squamous carcinoma cells". Oncology Reports 21, no. 2 (2009): 461-465. https://doi.org/10.3892/or_00000245