Avemar, a nontoxic fermented wheat germ extract, attenuates the growth of sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells through induction of apoptosis

  • Authors:
    • Philipp Saiko
    • Maria Ozsvar-Kozma
    • Geraldine Graser
    • Andreas Lackner
    • Michael Grusch
    • Sibylle Madlener
    • Georg Krupitza
    • Walter Jaeger
    • Mate Hidvegi
    • Ram P. Agarwal
    • Monika Fritzer-Szekeres
    • Thomas Szekeres
  • View Affiliations

  • Published online on: March 1, 2009     https://doi.org/10.3892/or_00000285
  • Pages: 787-791
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Avemar (MSC) is a nontoxic fermented wheat germ extract, which has been shown to significantly improve the survival rate in patients suffering from various malignancies. We investigated its effects in sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells. After 48 and 72 h of incubation, Avemar inhibited the growth of sensitive H9 cells with IC50 values of 290 and 200 µg/ml, whereas the growth of 5-FdUrd/Ara-C cross-resistant H9 cells was attenuated with IC50 values of 180 and 145 µg/ml, respectively. Treatment with 300 µg/ml MSC for 48 h caused dose-dependent induction of apoptosis in 48% of sensitive H9 cells. In cross-resistant H9 cells, incubation with 200 µg/ml Avemar for 48 h led to 41% of apoptotic tumor cells. Growth arrest of sensitive H9 cells after exposure to various concentrations of MSC occurred mainly in the S phase of the cell cycle, thereby increasing the cell population from 54 to 73% while depleting cells in the G0-G1 phase from 40 to 19%. Growth arrest in cross-resistant H9 cells occurred also mainly in the S phase, increasing the cell population from 45 to 68% while depleting cells in the G0-G1 phase from 45 to 31%. As MSC treatment likely overcomes 5-FdUrd/Ara-C resistance, further investigations to elucidate the exact mechanisms are warranted. We conclude that Avemar exerts a number of beneficial effects which could support conventional chemotherapy of human malignancies.

Related Articles

Journal Cover

March 2009
Volume 21 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Saiko P, Ozsvar-Kozma M, Graser G, Lackner A, Grusch M, Madlener S, Krupitza G, Jaeger W, Hidvegi M, Agarwal RP, Agarwal RP, et al: Avemar, a nontoxic fermented wheat germ extract, attenuates the growth of sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells through induction of apoptosis. Oncol Rep 21: 787-791, 2009.
APA
Saiko, P., Ozsvar-Kozma, M., Graser, G., Lackner, A., Grusch, M., Madlener, S. ... Szekeres, T. (2009). Avemar, a nontoxic fermented wheat germ extract, attenuates the growth of sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells through induction of apoptosis. Oncology Reports, 21, 787-791. https://doi.org/10.3892/or_00000285
MLA
Saiko, P., Ozsvar-Kozma, M., Graser, G., Lackner, A., Grusch, M., Madlener, S., Krupitza, G., Jaeger, W., Hidvegi, M., Agarwal, R. P., Fritzer-Szekeres, M., Szekeres, T."Avemar, a nontoxic fermented wheat germ extract, attenuates the growth of sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells through induction of apoptosis". Oncology Reports 21.3 (2009): 787-791.
Chicago
Saiko, P., Ozsvar-Kozma, M., Graser, G., Lackner, A., Grusch, M., Madlener, S., Krupitza, G., Jaeger, W., Hidvegi, M., Agarwal, R. P., Fritzer-Szekeres, M., Szekeres, T."Avemar, a nontoxic fermented wheat germ extract, attenuates the growth of sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells through induction of apoptosis". Oncology Reports 21, no. 3 (2009): 787-791. https://doi.org/10.3892/or_00000285