Aspirin attenuates angiotensin II‑induced inflammation in bone marrow mesenchymal stem cells via the inhibition of ERK1/2 and NF‑κB activation

  • Authors:
    • Fenxi Zhang
    • Ming Lu
    • Huaibin Wang
    • Tongming Ren
  • View Affiliations

  • Published online on: August 23, 2013     https://doi.org/10.3892/br.2013.160
  • Pages: 930-934
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Angiotensin II (Ang II) is a peptide hormone that plays a critical role in numerous physiological and pathophysiological processes. It is also commonly used as an inducer for the directional differentiation of bone marrow mesenchymal stem cells (bmMSCs). Previous studies demonstrated that Ang II induces inflammatory responses in endothelial cells, smooth muscle cells and fibroblasts. Aspirin is generally used as analgesic, antipyretic and occasionally anti‑inflammatory medication. Whether aspirin suppresses inflammatory responses in bmMSCs has not been elucidated. In this study, we investigated the effect of aspirin on Ang II‑induced inflammation in bmMSCs. Our results demonstrated that Ang II (10 nM‑10 µM) increased the secretion of tumor necrosis factor (TNF)‑α and interleukin (IL)‑6 from bmMSCs in a dose‑dependent manner. This result was further confirmed by a reverse transcription‑polymerase chain reaction (RT‑PCR) assay, which demonstrated a dose‑dependent increase in the mRNA expression of TNF‑α, IL‑6, IL‑1β and monocyte chemotactic protein‑1 (MCP‑1) in bmMSCs following exposure to Ang II. Furthermore, it was also observed that Ang II increased the expression of phospho‑extracellular signal‑regulated kinase 1/2 (ERK1/2) and phospho‑nuclear factor κ‑light‑chain‑enhancer of activated B cells (NF‑κB)‑p65 in bmMSCs. The application of aspirin (0.1 mM) significantly inhibited the activation of ERK1/2 and NF‑κB, the expression of TNF‑α, IL‑6, IL‑1β and MCP‑1 genes and the secretion of TNF‑α and IL‑6. Our findings indicated that aspirin may attenuate Ang II‑induced inflammation in bmMSCs via the inhibition of ERK1/2 and NF‑κB activation.
View Figures
View References

Related Articles

Journal Cover

November-December 2013
Volume 1 Issue 6

Print ISSN: 2049-9434
Online ISSN:2049-9442

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang F, Lu M, Wang H and Ren T: Aspirin attenuates angiotensin II‑induced inflammation in bone marrow mesenchymal stem cells via the inhibition of ERK1/2 and NF‑κB activation. Biomed Rep 1: 930-934, 2013
APA
Zhang, F., Lu, M., Wang, H., & Ren, T. (2013). Aspirin attenuates angiotensin II‑induced inflammation in bone marrow mesenchymal stem cells via the inhibition of ERK1/2 and NF‑κB activation. Biomedical Reports, 1, 930-934. https://doi.org/10.3892/br.2013.160
MLA
Zhang, F., Lu, M., Wang, H., Ren, T."Aspirin attenuates angiotensin II‑induced inflammation in bone marrow mesenchymal stem cells via the inhibition of ERK1/2 and NF‑κB activation". Biomedical Reports 1.6 (2013): 930-934.
Chicago
Zhang, F., Lu, M., Wang, H., Ren, T."Aspirin attenuates angiotensin II‑induced inflammation in bone marrow mesenchymal stem cells via the inhibition of ERK1/2 and NF‑κB activation". Biomedical Reports 1, no. 6 (2013): 930-934. https://doi.org/10.3892/br.2013.160