4-Nitroquinoline-1-oxide effects human lung adenocarcinoma A549 cells by regulating the expression of POLD4

  • Authors:
    • Qin‑Miao Huang
    • Yi‑Ming Zeng
    • Hua‑Ping Zhang
    • Liang‑Chao Lv
    • Dong‑Yong Yang
    • Hui‑Huang Lin
  • View Affiliations

  • Published online on: January 26, 2016     https://doi.org/10.3892/br.2016.583
  • Pages: 345-348
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Abstract

The aim of the present study was to explore the expression of POLD4 in human lung adenocarcinoma A549 cells under 4-nitroquinoline-1-oxide (4NQO) stimulation to investigate the role of POLD4 in smoking-induced lung cancer. The lung cancer A549 cell line was treated with 4NQO, with or without MG132 (an inhibitor of proteasome activity), and subsequently the POLD4 level was determined by western blot analysis. Secondly, the cell sensitivity to 4NQO and Taxol was determined when the POLD4 expression level was downregulated by siRNA. The POLD4 protein levels in the A549 cells decreased following treatment with 4NQO; however, MG132 could reverse this phenotype. Downregulation of the POLD4 expression by siRNA enhanced A549 cell sensitivity to 4NQO, but not to Taxol. In conclusion, 4NQO affects human lung adenocarcinoma A549 cells by regulating the expression of POLD4.
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March-2016
Volume 4 Issue 3

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Huang QM, Zeng YM, Zhang HP, Lv LC, Yang DY and Lin HH: 4-Nitroquinoline-1-oxide effects human lung adenocarcinoma A549 cells by regulating the expression of POLD4. Biomed Rep 4: 345-348, 2016
APA
Huang, Q., Zeng, Y., Zhang, H., Lv, L., Yang, D., & Lin, H. (2016). 4-Nitroquinoline-1-oxide effects human lung adenocarcinoma A549 cells by regulating the expression of POLD4. Biomedical Reports, 4, 345-348. https://doi.org/10.3892/br.2016.583
MLA
Huang, Q., Zeng, Y., Zhang, H., Lv, L., Yang, D., Lin, H."4-Nitroquinoline-1-oxide effects human lung adenocarcinoma A549 cells by regulating the expression of POLD4". Biomedical Reports 4.3 (2016): 345-348.
Chicago
Huang, Q., Zeng, Y., Zhang, H., Lv, L., Yang, D., Lin, H."4-Nitroquinoline-1-oxide effects human lung adenocarcinoma A549 cells by regulating the expression of POLD4". Biomedical Reports 4, no. 3 (2016): 345-348. https://doi.org/10.3892/br.2016.583