Overexpression of suppressor of IKBKE 1 is associated with vincristine resistance in colon cancer cells

Zhao,Chun‑Peng; Xu,Zhong‑Jie; Guo,Qing; Li,Yun‑Xiao; Gao,Xiang‑Zheng; Peng,Yi‑You

doi:10.3892/br.2016.759

Overexpression of suppressor of IKBKE 1 is associated with vincristine resistance in colon cancer cells

Authors:
- Chun‑Peng Zhao
- Zhong‑Jie Xu
- Qing Guo
- Yun‑Xiao Li
- Xiang‑Zheng Gao
- Yi‑You Peng
View Affiliations

Affiliations: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Life Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of College of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
Published online on: September 21, 2016 https://doi.org/10.3892/br.2016.759
Pages: 585-588

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

In a previous study, the suppressor of IKBKE 1 expression level was confirmed to be higher in vincristine (VCR)‑resistant HCT‑8 (HCT‑8/V) colon cancer cells than in non‑VCR‑resistant HCT‑8 cells. In the current study, IKBKE 1 expression in VCR‑resistant colon cancer cells was investigated further. HCT‑8 and HCT‑8/V human colon cancer cells were used, and polymerase chain reaction (PCR) primers were designed to amplify the IKBKE 1 gene. Fluorescence reverse transcription‑quantitative PCR (RT‑qPCR) was performed to detect differences in IKBKE 1 expression between sensitive and drug‑resistant colon cancer cell lines. Western blotting was performed to further observe IKBKE 1 expression. Based on the RT‑qPCR and western blot results, IKBKE 1 expression was observed to be markedly higher in the HCT‑8/V cells, and this difference was significant (P<0.05). Thus, IKBKE 1 expression was identified to be associated with the resistance of colon cancer cells to VCR.

View Figures

View References

1	Kim JH: Chemotherapy for colorectal cancer in the elderly. World J Gastroenterol. 21:5158–5166. 2015. View Article : Google Scholar : PubMed/NCBI
2	Davidson M, Okines AF and Starling N: Current and Future Therapies for Advanced Gastric Cancer. Clin Colorectal Cancer. 14:239–250. 2015. View Article : Google Scholar : PubMed/NCBI
3	Zhang L, Ngo JA, Wetzel MD and Marchetti D: Heparanase mediates a novel mechanism in lapatinib-resistant brain metastatic breast cancer. Neoplasia. 17:101–113. 2015. View Article : Google Scholar : PubMed/NCBI
4	Jin AH and Wei ZL: Molecular mechanism of increased sensitivity of cisplatin to ovarian cancer by inhibition of microRNA-23a expression. Int J Clin Exp Med. 8:13329–13334. 2015.PubMed/NCBI
5	Wu Z, Zhang Z, Ge X, Lin Y, Dai C, Chang J, Liu X, Geng R, Wang C, Chen H, et al: Identification of short-form RON as a novel intrinsic resistance mechanism for anti-MET therapy in MET-positive gastric cancer. Oncotarget. 6:40519–40534. 2015.PubMed/NCBI
6	Ho CM, Huang CJ, Huang SH, Chang SF and Cheng WF: Demethylation of HIN-1 reverses paclitaxel-resistance of ovarian clear cell carcinoma through the AKT-mTOR signaling pathway. BMC Cancer. 15:7892015. View Article : Google Scholar : PubMed/NCBI
7	Jung SO, Kim SY, Kim JO, Jung SS, Park HS, Moon JY, Kim SM and Lee JE: Promising effects of 3rd line cyclophosphamide, adriamycin and vincristine (CAV) and 4th line ifosfamide and carboplatin chemotherapy in refractory small cell lung cancer. Thorac Cancer. 6:659–663. 2015. View Article : Google Scholar : PubMed/NCBI
8	Chau TL, Gioia R, Gatot JS, Patrascu F, Carpentier I, Chapelle JP, O'Neill L, Beyaert R, Piette J and Chariot A: Are the IKKs and IKK-related kinases TBK1 and IKK-epsilon similarly activated? Trends Biochem Sci. 33:171–180. 2008. View Article : Google Scholar : PubMed/NCBI
9	Huang J, Liu T, Xu LG, Chen D, Zhai Z and Shu HB: SIKE is an IKK epsilon/TBK1-associated suppressor of TLR3- and virus-triggered IRF-3 activation pathways. EMBO J. 24:4018–4028. 2005. View Article : Google Scholar : PubMed/NCBI
10	Hsieh JY, Huang TS, Cheng SM, Lin WS, Tsai TN, Lee OK and Wang HW: miR-146a-5p circuitry uncouples cell proliferation and migration, but not differentiation, in human mesenchymal stem cells. Nucleic Acids Res. 41:9753–9763. 2013. View Article : Google Scholar : PubMed/NCBI
11	Sun QL, Zhao CP, Wang TY, Hao XB, Wang XY, Zhang X and Li YC: Expression profile analysis of long non-coding RNA associated with vincristine resistance in colon cancer cells by next-generation sequencing. Gene. 572:79–86. 2015. View Article : Google Scholar : PubMed/NCBI
12	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2⁻ΔΔ^Ct Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
13	Dong WH, Wang TY, Wang F and Zhang JH: Simple, time-saving dye staining of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue. PLoS One. 6:e223942011. View Article : Google Scholar : PubMed/NCBI
14	Chao MW, Lai MJ, Liou JP, Chang YL, Wang JC, Pan SL and Teng CM: The synergic effect of vincristine and vorinostat in leukemia in vitro and in vivo. J Hematol Oncol. 8:822015. View Article : Google Scholar : PubMed/NCBI
15	Lewis RS, Fidel J, Dassanayake S, Court MH, Burke NS and Mealey KL: Comparison of chemotherapeutic drug resistance in cells transfected with canine ABCG2 or feline ABCG2. Vet Comp Oncol. Oct 14–2015.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI
16	Xu Y and Qiu L: Nonspecifically enhanced therapeutic effects of vincristine on multidrug-resistant cancers when coencapsulated with quinine in liposomes. Int J Nanomedicine. 10:4225–4237. 2015.PubMed/NCBI
17	Martins A, Sipos P, Dér K, Csábi J, Miklos W, Berger W, Zalatnai A, Amaral L, Molnár J, Szabó-Révész P and Hunyadi A: Ecdysteroids sensitize MDR and non-MDR cancer cell lines to doxorubicin, paclitaxel, and vincristine but tend to protect them from cisplatin. Biomed Res Int. 2015:8953602015. View Article : Google Scholar : PubMed/NCBI
18	Bartram I, Erben U, Ortiz-Tanchez J, Blunert K, Schlee C, Neumann M, Heesch S and Baldus CD: Inhibition of IGF1-R overcomes IGFBP7-induced chemotherapy resistance in T-ALL. BMC Cancer. 15:6632015. View Article : Google Scholar : PubMed/NCBI
19	Tivnan A, Zakaria Z, O'Leary C, Kögel D, Pokorny JL, Sarkaria JN and Prehn JH: Inhibition of multidrug resistance protein 1 (MRP1) improves chemotherapy drug response in primary and recurrent glioblastoma multiforme. Front Neurosci. 9:2182015. View Article : Google Scholar : PubMed/NCBI
20	Tsubaki M, Takeda T, Ogawa N, Sakamoto K, Shimaoka H, Fujita A, Itoh T, Imano M, Ishizaka T, Satou T, et al: Overexpression of survivin via activation of ERK1/2, Akt, and NF-κB plays a central role in vincristine resistance in multiple myeloma cells. Leuk Res. 39:445–452. 2015. View Article : Google Scholar : PubMed/NCBI
21	Moqadam F Akbari, Lange-Turenhout EA, Ariës IM, Pieters R and den Boer ML: miR-125b, miR-100 and miR-99a co-regulate vincristine resistance in childhood acute lymphoblastic leukemia. Leuk Res. 37:1315–1321. 2013. View Article : Google Scholar : PubMed/NCBI
22	Wang TY, Zhang QQ, Zhang X, Sun QL, Zhao CP and Wang XY: The effect of recombinant lentiviral vector encoding miR-145 on human esophageal cancer cells. Tumour Biol. 36:9733–9738. 2015. View Article : Google Scholar : PubMed/NCBI
23	Xu Y, Xia F, Ma L, Shan J, Shen J, Yang Z, Liu J, Cui Y, Bian X, Bie P, et al: MicroRNA-122 sensitizes HCC cancer cells to adriamycin and vincristine through modulating expression of MDR and inducing cell cycle arrest. Cancer Lett. 310:160–169. 2011.PubMed/NCBI
24	Méndez C, Ahlenstiel CL and Kelleher AD: Post-transcriptional gene silencing, transcriptional gene silencing and human immunodeficiency virus. World J Virol. 4:219–244. 2015. View Article : Google Scholar : PubMed/NCBI