Molecular cloning, expression, purification and functional characterization of an antifungal cyclophilin protein from Panax ginseng

Zhang,Hui; Wang,Jiawen; Li,Shuaijun; Wang,Siming; Liu,Meichen; Wang,Weinan; Zhao,Yu

doi:10.3892/br.2017.998

Molecular cloning, expression, purification and functional characterization of an antifungal cyclophilin protein from Panax ginseng

Authors:
- Hui Zhang
- Jiawen Wang
- Shuaijun Li
- Siming Wang
- Meichen Liu
- Weinan Wang
- Yu Zhao
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Affiliations: Traditional Chinese Medicine and Biotechnology Research and Development Center, Changchun University of Traditional Chinese Medicine, Changchun, Jilin 130117, P.R. China, School of Pharmaceutical Sciences, Changchun University of Traditional Chinese Medicine, Changchun, Jilin 130117, P.R. China
Published online on: October 10, 2017 https://doi.org/10.3892/br.2017.998
Pages: 527-531
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cyclophilins (CyPs), a member of peptidyl‑prolyl cis‑trans isomerases (PPIases), are ubiquitously distributed in organisms such as bacteria, yeast, plants and animals. CyPs have diverse biological functions, with some exhibiting antifungal and antiviral activities. In this study, Panax ginseng cyclophilin (pgCyP), a novel gene encoding an antifungal protein from Panax ginseng, was cloned, and its protein product was expressed in Escherichia coli, and then fractionated by affinity chromatography. The open reading frame of the pgCyP full‑length coding sequence was found to encode a single‑domain CyP‑like protein of 174 amino residues with a calculated molecular weight of 18.7 kDa. The pGEX system was used to express pgCyP fused to glutathione S‑transferase. After affinity purification, the protein showed a strong fungal resistance effect on Phytophthora cactorum. In addition, pgCyP showed high PPIase activity. To the best of our knowledge, the present study is the first successful effort to clone and characterize a CyP‑like protein gene from Panax ginseng.

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