Repression of IGF-I-induced osteoblast migration by (-)-epigallocatechin gallate through p44/p42 MAP kinase signaling

Kawabata,Tetsu; Tokuda,Haruhiko; Sakai,Go; Fujita,Kazuhiko; Matsushima-Nishiwaki,Rie; Otsuka,Takanobu; Kozawa,Osamu

doi:10.3892/br.2018.1140

Repression of IGF-I-induced osteoblast migration by (-)-epigallocatechin gallate through p44/p42 MAP kinase signaling

Authors:
- Tetsu Kawabata
- Haruhiko Tokuda
- Go Sakai
- Kazuhiko Fujita
- Rie Matsushima-Nishiwaki
- Takanobu Otsuka
- Osamu Kozawa
View Affiliations

Affiliations: Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi 467-8601, Japan, Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan
Published online on: August 8, 2018 https://doi.org/10.3892/br.2018.1140
Pages: 318-326

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Polyphenolic compounds in beverages may have benefits in the prevention of osteoporosis. It has been demonstrated previously that insulin-like growth factor‑I (IGF-I) could stimulate the migration of osteoblasts. In the present study, it was investigated whether chlorogenic acid, a major polyphenol in coffee, and (-)-epigallocatechin gallate (EGCG), a major polyphenol in green tea, could affect this IGF-I‑stimulated migration of osteoblast-like MC3T3-E1 cells. The IGF-I‑stimulated osteoblast migration, evaluated by Transwell cell migration and wound-healing assays, was inhibited by EGCG but not chlorogenic acid. IGF-I induced the phosphorylation of p44/p42 mitogen-activated protein (MAP) kinase, p70 S6 kinase and Akt. The IGF-I-induced migration was suppressed by PD98059, a MAP kinase kinase 1/2 inhibitor, and deguelin, an Akt inhibitor, but not rapamycin, an inhibitor of the upstream kinase of p70 S6 kinase (mammalian target of rapamycin). EGCG attenuated the IGF-I-induced phosphorylation of p44/p42 MAP kinase but not Akt. Taken together, the present results suggest that EGCG inhibits IGF-I‑induced osteoblast migration via p44/p42 MAP kinase.

View Figures

View References

1	Karsenty G and Wagner EF: Reaching a genetic and molecular understanding of skeletal development. Dev Cell. 2:389–406. 2002. View Article : Google Scholar : PubMed/NCBI
2	Kular J, Tickner J, Chim SM and Xu J: An overview of the regulation of bone remodelling at the cellular level. Clin Biochem. 45:863–873. 2012. View Article : Google Scholar : PubMed/NCBI
3	Khan SN, Bostrom MP and Lane JM: Bone growth factors. Orthop Clin North Am. 31:375–388. 2000. View Article : Google Scholar : PubMed/NCBI
4	Lieberman JR, Daluiski A and Einhorn TA: The role of growth factors in the repair of bone. Biology and clinical applications. J Bone Joint Surg Am. 84-A:1–1044. 2002.PubMed/NCBI
5	Reddi AH, Roodman D, Freeman C and Mohla S: Mechanisms of tumor metastasis to the bone: Challenges and opportunities. J Bone Miner Res. 18:190–194. 2003. View Article : Google Scholar : PubMed/NCBI
6	Clemens TL and Chernausek SD: Genetic strategies for elucidating insulin-like growth factor action in bone. Growth Horm IGF Res. 14:195–199. 2004. View Article : Google Scholar : PubMed/NCBI
7	Niu T and Rosen CJ: The insulin-like growth factor-I gene and osteoporosis: A critical appraisal. Gene. 361:38–56. 2005. View Article : Google Scholar : PubMed/NCBI
8	Noda T, Tokuda H, Yoshida M, Yasuda E, Hanai Y, Takai S and Kozawa O: Possible involvement of phosphatidylinositol 3-kinase/Akt pathway in insulin-like growth factor-I-induced alkaline phosphatase activity in osteoblasts. Horm Metab Res. 37:270–274. 2005. View Article : Google Scholar : PubMed/NCBI
9	Hanai Y, Tokuda H, Ishisaki A, Matsushima-Nishiwaki R, Nakamura N, Yoshida M, Takai S, Ohta T and Kozawa O: Involvement of p44/p42 MAP kinase in insulin-like growth factor-I-induced alkaline phosphatase activity in osteoblast-like-MC3T3-E1 cells. Mol Cell Endocrinol. 251:42–48. 2006. View Article : Google Scholar : PubMed/NCBI
10	Nakasaki M, Yoshioka K, Miyamoto Y, Sasaki T, Yoshikawa H and Itoh K: IGF-I secreted by osteoblasts acts as a potent chemotactic factor for osteoblasts. Bone. 43:869–879. 2008. View Article : Google Scholar : PubMed/NCBI
11	Jankun J, Selman SH, Swiercz R and Skrzypczak-Jankun E: Why drinking green tea could prevent cancer. Nature. 387:5611997. View Article : Google Scholar : PubMed/NCBI
12	Koo SH and Montminy M: In vino veritas: A tale of two sirt1s? Cell. 127:1091–1093. 2006. View Article : Google Scholar : PubMed/NCBI
13	George SE, Ramalakshmi K and Mohan Rao LJ: A perception on health benefits of coffee. Crit Rev Food Sci Nutr. 48:464–486. 2008. View Article : Google Scholar : PubMed/NCBI
14	Thielecke F and Boschmann M: The potential role of green tea catechins in the prevention of the metabolic syndrome - a review. Phytochemistry. 70:11–24. 2009. View Article : Google Scholar : PubMed/NCBI
15	Shimizu M, Adachi S, Masuda M, Kozawa O and Moriwaki H: Cancer chemoprevention with green tea catechins by targeting receptor tyrosine kinases. Mol Nutr Food Res. 55:832–843. 2011. View Article : Google Scholar : PubMed/NCBI
16	Folwarczna J, Pytlik M, Zych M, Cegieła U, Nowinska B, Kaczmarczyk-Sedlak I, Sliwinski L, Trzeciak H and Trzeciak HI: Effects of caffeic and chlorogenic acids on the rat skeletal system. Eur Rev Med Pharmacol Sci. 19:682–693. 2015.PubMed/NCBI
17	Kwak SC, Lee C, Kim JY, Oh HM, So HS, Lee MS, Rho MC and Oh J: Chlorogenic acid inhibits osteoclast differentiation and bone resorption by down-regulation of receptor activator of nuclear factor κ-B ligand-induced nuclear factor of activated T cells c1 expression. Biol Pharm Bull. 36:1779–1786. 2013. View Article : Google Scholar : PubMed/NCBI
18	Shen CL, Yeh JK, Cao JJ and Wang JS: Green tea and bone metabolism. Nutr Res. 29:437–456. 2009. View Article : Google Scholar : PubMed/NCBI
19	Singh R, Akhtar N and Haqqi TM: Green tea polyphenol epigallocatechin-3-gallate: Inflammation and arthritis. [corrected]. Life Sci. 86:907–918. 2010. View Article : Google Scholar : PubMed/NCBI
20	Fujita K, Otsuka T, Yamamoto N, Kainuma S, Ohguchi R, Kawabata T, Sakai G, Kuroyanagi G, Matsushima-Nishiwaki R, Kozawa O, et al: (−)-Epigallocatechin gallate but not chlorogenic acid upregulates osteoprotegerin synthesis through regulation of bone morphogenetic protein-4 in osteoblasts. Exp Ther Med. 14:417–423. 2017. View Article : Google Scholar : PubMed/NCBI
21	Sudo H, Kodama HA, Amagai Y, Yamamoto S and Kasai S: In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol. 96:191–198. 1983. View Article : Google Scholar : PubMed/NCBI
22	Kozawa O, Tokuda H, Miwa M, Kotoyori J and Oiso Y: Cross-talk regulation between cyclic AMP production and phosphoinositide hydrolysis induced by prostaglandin E2 in osteoblast-like cells. Exp Cell Res. 198:130–134. 1992. View Article : Google Scholar : PubMed/NCBI
23	Karagiosis SA, Chrisler WB, Bollinger N and Karin NJ: Lysophosphatidic acid-induced ERK activation and chemotaxis in MC3T3-E1 preosteoblasts are independent of EGF receptor transactivation. J Cell Physiol. 219:716–723. 2009. View Article : Google Scholar : PubMed/NCBI
24	Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970. View Article : Google Scholar : PubMed/NCBI
25	Kato K, Ito H, Hasegawa K, Inaguma Y, Kozawa O and Asano T: Modulation of the stress-induced synthesis of hsp27 and alpha B-crystallin by cyclic AMP in C6 rat glioma cells. J Neurochem. 66:946–950. 1996. View Article : Google Scholar : PubMed/NCBI
26	Kyriakis JM and Avruch J: Mammalian MAPK signal transduction pathways activated by stress and inflammation: A 10-year update. Physiol Rev. 92:689–737. 2012. View Article : Google Scholar : PubMed/NCBI
27	Ip CK, Cheung AN, Ngan HY and Wong AS: p70 S6 kinase in the control of actin cytoskeleton dynamics and directed migration of ovarian cancer cells. Oncogene. 30:2420–2432. 2011. View Article : Google Scholar : PubMed/NCBI
28	Alessi DR, Cuenda A, Cohen P, Dudley DT and Saltiel AR: PD 098059 is a specific inhibitor of the activation of mitogen-activated protein kinase kinase in vitro and in vivo. J Biol Chem. 270:27489–27494. 1995. View Article : Google Scholar : PubMed/NCBI
29	Li Y, Corradetti MN, Inoki K and Guan KL: TSC2: Filling the GAP in the mTOR signaling pathway. Trends Biochem Sci. 29:32–38. 2004. View Article : Google Scholar : PubMed/NCBI
30	Toker A and Newton AC: Cellular signaling: Pivoting around PDK-1. Cell. 103:185–188. 2000. View Article : Google Scholar : PubMed/NCBI
31	Shen CL, Wang P, Guerrieri J, Yeh JK and Wang JS: Protective effect of green tea polyphenols on bone loss in middle-aged female rats. Osteoporos Int. 19:979–990. 2008. View Article : Google Scholar : PubMed/NCBI
32	Mah YJ, Song JS, Kim SO, Lee JH, Jeon M, Jung UW, Moon SJ, Kim JH and Choi HJ: The effect of epigallocatechin-3-gallate (EGCG) on human alveolar bone cells both in vitro and in vivo. Arch Oral Biol. 59:539–549. 2014. View Article : Google Scholar : PubMed/NCBI
33	Yang CS, Chen L, Lee MJ, Balentine D, Kuo MC and Schantz SP: Blood and urine levels of tea catechins after ingestion of different amounts of green tea by human volunteers. Cancer Epidemiol Biomarkers Prev. 7:351–354. 1998.PubMed/NCBI
34	Stalmach A, Williamson G and Crozier A: Impact of dose on the bioavailability of coffee chlorogenic acids in humans. Food Funct. 5:1727–1737. 2014. View Article : Google Scholar : PubMed/NCBI
35	Zhu Z, Huang P, Chong Y, George SK, Wen B, Han N, Liu Z, Kang L and Lin N: Nucleus pulposus cells derived IGF-1 and MCP-1 enhance osteoclastogenesis and vertebrae disruption in lumbar disc herniation. Int J Clin Exp Pathol. 7:8520–8531. 2014.PubMed/NCBI