Antiviral effects of dehydroascorbic acid

Uozaki,Misao; Ikeda,Keiko; Tsujimoto,Kazuko; Nishide,Mitsunori; Yamasaki,Hisashi; Khamsri,Boonruang; Hajime Koyama,A.

doi:10.3892/etm.2010.139

Antiviral effects of dehydroascorbic acid

Authors:
- Misao Uozaki
- Keiko Ikeda
- Kazuko Tsujimoto
- Mitsunori Nishide
- Hisashi Yamasaki
- Boonruang Khamsri
- A. Hajime Koyama
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Affiliations: Division of Virology, Department of Cellular and Molecular Medicine, Wakayama Medical University Graduate School of Medicine, Wakayama 641-0011, Japan, Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand
Published online on: August 26, 2010 https://doi.org/10.3892/etm.2010.139
Pages: 983-986

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Abstract

In the present study, dehydroascorbic acid inhibited the multiplication of viruses of three different families: herpes simplex virus type 1 (HSV-1), influenza virus type A and poliovirus type 1. Although dehydroascorbic acid showed some cytotoxicity at higher concentrations, the observed antiviral activity was not the secondary result of the cytotoxic effect of the reagent, as the inhibition of virus multiplication was observed at reagent concentrations significantly lower than those resulting in cytotoxicity. Characterization of the mode of the antiviral action of dehydroascorbic acid against HSV-1 revealed that the addition of reagent at any time post infection inhibited the formation of progeny infectious virus in the infected cells, and a one-step growth curve showed that the addition of reagent allowed formation for an additional 2 h, but then almost completely suppressed it. These results indicate that the reagent inhibits HSV-1 multiplication after the completion of viral DNA replication, probably at the step of the envelopment of viral nucleocapsids at the Golgi apparatus of infected cells.

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