Petroleum ether extract of Chenopodium album L. prevents cell growth and induces apoptosis of human lung cancer cells

Zhao,Ting; Pan,Hui; Feng,Yang; Li,Haizhou; Zhao,Yang

doi:10.3892/etm.2016.3765

Petroleum ether extract of Chenopodium album L. prevents cell growth and induces apoptosis of human lung cancer cells

Authors:
- Ting Zhao
- Hui Pan
- Yang Feng
- Haizhou Li
- Yang Zhao
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Affiliations: Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan 650500, P.R. China, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, P.R. China
Published online on: October 3, 2016 https://doi.org/10.3892/etm.2016.3765
Pages: 3301-3307
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chenopodium album L. is a common edible herb distributed in China that has been used as a traditional Chinese medicine for antiviral, antifungal, anti‑inflammatory and cancer treatment. However, to the best of our knowledge no previous reports have investigated its the function of its phytochemical extracts in lung cancer cells. The purpose of the present study was to assess the anticancer activities of the phytochemical extracts of C. album L. on human non‑small cell lung cancer A549 cells. The present findings demonstrated that the petroleum ether (PE) extract of C. album L. exhibited significant growth inhibitory effects on A549 with an IC50 value of 33.31±2.79 µg/ml. As determined by MTT and colony formation assays, its growth inhibitory effects were dose‑ and time‑dependent. Furthermore, PE extract‑treated A549 cells exhibited dose‑dependent cell growth arrest at the G1 phase of the cell cycle and cell apoptosis was induced. These results provide useful data on the anticancer activities of C. album L. in human lung cancer and demonstrated the novel possibilities of this plant in developing lung cancer therapies.

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1	Siegel R, Naishadham D and Jemal A: Cancer statistics. Cancer J Clin. 62:10–29. 2013. View Article : Google Scholar
2	She J, Yang P, Hong QY and Bai CX: Lung cancer in China: Challenges and interventions. Chest. 143:1117–1126. 2013. View Article : Google Scholar : PubMed/NCBI
3	Verdeccchia A, Francisci S, Brenner H, Gatta G, Micheli A, Mangone L and Kunkler I: EUROCARE-4 Working Group: Recent cancer survival in Europe: A 2000-02 period analysis of EUROCARE-4 data. Lancet Oncol. 8:784–796. 2007. View Article : Google Scholar : PubMed/NCBI
4	Clardy J and Walsh C: Lesson from natural molecules. Nature. 432:829–837. 2004. View Article : Google Scholar : PubMed/NCBI
5	Piggott AM and Karuso P: Quality, not quantity: The role of natural products and chemical proteomics in modern drug discovery. Comb Chem High Throughput Screen. 7:607–630. 2004. View Article : Google Scholar : PubMed/NCBI
6	Koch MA, Schuffenhauer A, Scheck M, Wetzel S, Casaulta S, Odermatt A, Ertl P and Waldmann H: Charting biologically relevant chemical space: A structural classification of natural products (SCONP). Proc Natl Acad Sci USA. 102:17272–17277. 2005. View Article : Google Scholar : PubMed/NCBI
7	Hao XN, Chan SW and Chen SL: Detection of Puerarin and Danshensu in traditional Chinese medicinal preparation containing Pueraria lobata and Salvia Miltiorrhiza by high-performance liquid chromatography. J Liq Chromatogr Relat Technol. 30:2779–2787. 2007. View Article : Google Scholar
8	Hong JY, Nam JW, Seo EK and Lee SK: Daphnane diterpene esters with anti-proliferative activities against human lung cancer cells from Daphne genkwa. Chem Pharm Bull (Tokyo). 58:234–237. 2010. View Article : Google Scholar : PubMed/NCBI
9	Toyang NJ, Ateh EN, Davis H, Tane P, Sondengam LB, Bryant J and Verpoorte R: In vivo antiprostate tumor potential of Vernonia guineensis Benth.(Asteraceae) tuber extract (VGDE) and the cytotoxicity of its major compound pentaisovaleryl sucrose. J Ethnopharmacol. 150:724–748. 2013. View Article : Google Scholar : PubMed/NCBI
10	Wang DS, Rizwani GH, Guo H, Ahmed M, Ahmed M, Hassan SZ, Hassan A, Chen ZS and Xu RH: Annona squamosa Linn: Cytotoxic activity found in leaf extract against human tumor cell lines. Pak J Pharm Sci. 27:1559–1563. 2014.PubMed/NCBI
11	Kumar R, Mishra AK, Dubey NK and Tripathi YB: Evaluation of Chenopodium ambrosioides oil as a potential source of antifungal, antiaflatoxigenic and antioxidant activity. Int J Food Microbial. 115:159–164. 2007. View Article : Google Scholar
12	Kaur C and Kapoor HC: Antioxidant activity and total phenolic content of some Asian vegetables. J Food Sci Technol. 37:153–161. 2002. View Article : Google Scholar
13	Dai Y, Ye WC, Wang ZT, Matsuda H, Kubo M and But PPH: Antipruritic and antinociceptive effects of Chenopodium album L. in mice. J Ethnopharmacol. 81:245–250. 2002. View Article : Google Scholar : PubMed/NCBI
14	Khoobchandani M, Ojeswi BK, Sharma B and Srivastava MM: Chenopodium album prevents progression of cell growth and enhances cell toxicity in human breast cancer cell lines. Oxid Med Cell Longev. 2:160–165. 2009. View Article : Google Scholar : PubMed/NCBI
15	Li T, Pan H, Feng Y, Li HZ and Zhao Y: Bioactivity-guided isolation of anticancer constituents from Hedera nepalensis K. S Afr J Bot. 100:87–93. 2015. View Article : Google Scholar
16	Cheng YL, Lee SC, Harn HJ, Huang HC and Chang WL: The extract of Hibiscus syriacus inducing apoptosis by activating p53 and AIF in human lung cancer cells. Am J Chin Med. 36:171–184. 2008. View Article : Google Scholar : PubMed/NCBI
17	Li WY, Chan SW, Guo DJ, Chung MK, Leung TY and Yu PH: Water extract of Rheum officinale Baill. induces apoptosis in human lung adenocarcinoma A549 and human breast cancer MCF-7 cell lines. J Ethnopharmacol. 124:251–256. 2009. View Article : Google Scholar : PubMed/NCBI
18	Qi F, Li A, Inagaki Y, Gao J, Li J, Kokudo N, Li XK and Tang W: Chinese herbal medicines as adjuvant treatment during chemo-or radio-therapy for cancer. Biosci Trends. 4:297–307. 2010.PubMed/NCBI
19	Choi KS: Autophagy and cancer. Exp Mol Med. 44:109–120. 2012. View Article : Google Scholar : PubMed/NCBI
20	Hu YL, Jahangiri A, Delay M and Aghi MK: Tumor cell autophagy as an adaptiveresponse mediating resistance to treatments such as antiangiogenic therapy. Cancer Res. 72:4294–4299. 2012. View Article : Google Scholar : PubMed/NCBI
21	Hanahan D and Weinberg RA: The hallmarks of cancer. Cell. 100:57–70. 2000. View Article : Google Scholar : PubMed/NCBI
22	Evan GI and Vousden KH: Proliferation, cell cycle and apoptosis in cancer. Nature. 411:342–348. 2001. View Article : Google Scholar : PubMed/NCBI
23	Hu H, Ahn NS, Yang X, Lee YS and Kang KS: Ganoderma lucidum extract induces cell cycle arrest and apoptosis in MCF-7 human breast cancer cell. Int J Cancer. 102:250–253. 2002. View Article : Google Scholar : PubMed/NCBI
24	Johnson VL, Ko SC, Holmstrom TH, Eriksson JE and Chow SC: Effector caspases are dispensable for the early nuclear morphological changes during chemical-induced apoptosis. J Cell Sci. 113:2941–2953. 2000.PubMed/NCBI
25	van Engeland M, Ramaekers FC, Schutte B and Reutelingsperger CP: A novel assay to measure loss of plasma membrane asymmetry during apoptosis of adherent cells in culture. Cytometry. 24:131–139. 1996. View Article : Google Scholar : PubMed/NCBI
26	Schultz DR and Harringto WJ Jr: Apoptosis: Programmed cell death at molecular level. Semin Arthritis Rheum. 32:345–369. 2003. View Article : Google Scholar : PubMed/NCBI
27	Lowe SW and Lin AW: Apoptosis in cancer. Carcinogenesis. 21:485–495. 2000. View Article : Google Scholar : PubMed/NCBI
28	Ong CS, Zhou J, Ong CN and Shen HM: Luteolin induces G1 arrest in human nasopharyngeal carcinoma cells via the Akt-GSK-3β-Cyclin D1 pathway. Cancer Lett. 298:167–175. 2010. View Article : Google Scholar : PubMed/NCBI
29	Pitchakarn P, Suzuki S, Ogawa K, Pompimon W, Takahashi S, Asamoto M, Limtrakul P and Shirai T: Induction of G1 arrest and apoptosis in androgen-dependent human prostate cancer by Kuguacin J, a triterpenoid from Momordica charantia leaf. Cancer Lett. 306:142–150. 2011. View Article : Google Scholar : PubMed/NCBI
30	Yano H, Mizoguchi A, Fukuda K, Haramaki M, Ogasawara S, Momosaki S and Kojiro M: The herbal medicine sho-saiko-to inhibits proliferation of cancer cell lines by inducing apoptosis and arrest at the G0/G1 phase. Cancer Res. 54:448–454. 1994.PubMed/NCBI
31	Li Y, Ma HL, Han L, Liu WY, Zhao BX, Zhang SL and Miao JY: Novel ferrocenyl derivatives exert anti-cancer effect in human lung cancer cells in vitro via inducing G1-phase arrest and senescence. Acta Pharmacol Sin. 34:960–968. 2013. View Article : Google Scholar : PubMed/NCBI
32	Gao J, Morgan WA, Sanchez-Medina A and Corcoran O: The ethanol extract of Scutellaria baicalensis and the active compounds induce cell cycle arrest and apoptosis including upregulation of p53 and Bax in human lung cancer cells. Toxicol Appl Pharmacol. 254:221–228. 2011. View Article : Google Scholar : PubMed/NCBI
33	Tsai JC, Tsai S and Chang WC: Effect of ethanol extracts of three Chinese medicinal plants with laxative properties on ion transport of the rat intestinal epithelia. Biol Pharm Bull. 27:162–165. 2004. View Article : Google Scholar : PubMed/NCBI
34	Martins S, Mussatto SI, Martínez-Avila G, Montañez-Saenz J, Aguilar CN and Teixeira JA: Bioactive phenolic compounds: Production and extraction by solid-state fermentation. A review. Biotechnol Adv. 29:365–373. 2011. View Article : Google Scholar : PubMed/NCBI
35	Bilgili SG, Akdeniz N, Akbayram S, Ceylan A, Çalka Ö and Karaman K: Phototoxic dermatitis due to Chenopodium album in a child. Pediatr Dermatol. 28:647–676. 2011. View Article : Google Scholar
36	Sun CH, Li Y, He HY, Du W and Cheng XF: Nutritive compositions of Chenopodium album and the evaluation as a vegetable resource. Guihaia. 25:589–601. 2005.