Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes

Gao,Shu‑Guang; Yu,Yang; Zeng,Chao; Lu,Shi‑Tao; Tian,Jian; Cheng,Chao; Li,Liang‑Jun; Lei,Guang‑Hua

doi:10.3892/etm.2016.3784

Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes

Authors:
- Shu‑Guang Gao
- Yang Yu
- Chao Zeng
- Shi‑Tao Lu
- Jian Tian
- Chao Cheng
- Liang‑Jun Li
- Guang‑Hua Lei
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Affiliations: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
Published online on: October 5, 2016 https://doi.org/10.3892/etm.2016.3784
Pages: 3488-3494

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Abstract

The aim of the present study was to investigate the effects of phosphorylated osteopontin (p-OPN) on apoptosis and pro-inflammatory cytokine expression in human knee osteoarthritis (OA) chondrocytes. Human knee OA chondrocytes obtained from patients who underwent total knee arthroplasty were treated with p‑OPN, OPN or buffer. Reverse transcription quantitative‑polymerase chain reaction (RT‑qPCR) and western blot analysis were used to assess the expression levels of proinflammatory factors, including interleukin (IL)‑1β, tumor necrosis factor (TNF)‑α, IL‑6 and nuclear factor (NF)‑κB. Apoptosis of human knee OA chondrocytes was detected by Annexin V-fluorescein isothiocyanate/propidium iodide flow cytometry. Compared with the controls, chondrocytes treated with OPN exhibited higher mRNA and protein expression levels of proinflammatory factors (IL‑1β, TNF‑α, IL‑6 and NF‑κB), and a higher percentage of apoptotic chondrocytes. Furthermore, chondrocytes treated with p‑OPN exhibited the highest mRNA and protein expression levels of proinflammatory factors (IL‑1β, TNF‑α, IL‑6, NF‑κB) and the highest percentage of apoptotic chondrocytes. p‑OPN induces chondrocyte apoptosis and proinflammatory factor release, which suggests that p‑OPN may contribute to OA pathogenesis, and inhibition of p‑OPN may provide a novel effective strategy to slow or halt OA progression.

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