Open Access

Cell penetrable‑mouse forkhead box P3 suppresses type 1 T helper cell‑mediated immunity in a murine model of delayed‑type hypersensitivity

  • Authors:
    • Xia Liu
    • Jun Wang
    • Hui Wang
    • Chen Zhou
    • Qihong Yu
    • Lei Yin
    • Weijiang Wu
    • Sheng Xia
    • Qixiang Shao
  • View Affiliations

  • Published online on: January 2, 2017     https://doi.org/10.3892/etm.2017.4020
  • Pages: 421-428
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Forkhead box P3 (FOXP3), which is a transcription factor, has a primary role in the development and function of regulatory T cells, and thus contributes to homeostasis of the immune system. A previous study generated a cell‑permeable fusion protein of mouse FOXP3 conjugated to a protein transduction domain (PTD‑mFOXP3) that successfully blocked differentiation of type 17 T helper cells in vitro and alleviated experimental arthritis in mice. In the present study, the role of PTD‑mFOXP3 in type 1 T helper (Th1) cell‑mediated immunity was investigated and the possible mechanisms for its effects were explored. Under Th1 polarization conditions, cluster of differentiation 4+ T cells were treated with PTD‑mFOXP3 and analyzed by flow cytometry in vitro, which revealed that PTD‑mFOXP3 blocked Th1 differentiation in vitro. Mice models of delayed type hypersensitivity (DTH) reactions were generated by subcutaneous sensitization and challenge with ovalbumin (OVA) to the ears of mice. PTD‑mFOXP3, which was administered via local subcutaneous injection, significantly reduced DTH‑induced inflammation, including ear swelling (ear swelling, P<0.001; pinnae weight, P<0.05 or P<0.01 with 0.25 and 1.25 mg/kg PTD‑mFOXP3, respectively), infiltration of T cells, and expression of interferon‑γ at local inflammatory sites (mRNA level P<0.05) compared with the DTH group. The results of the present study demonstrated that PTD‑mFOXP3 may attenuate DTH reactions by suppressing the infiltration and activity of Th1 cells.

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February-2017
Volume 13 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu X, Wang J, Wang H, Zhou C, Yu Q, Yin L, Wu W, Xia S and Shao Q: Cell penetrable‑mouse forkhead box P3 suppresses type 1 T helper cell‑mediated immunity in a murine model of delayed‑type hypersensitivity. Exp Ther Med 13: 421-428, 2017
APA
Liu, X., Wang, J., Wang, H., Zhou, C., Yu, Q., Yin, L. ... Shao, Q. (2017). Cell penetrable‑mouse forkhead box P3 suppresses type 1 T helper cell‑mediated immunity in a murine model of delayed‑type hypersensitivity. Experimental and Therapeutic Medicine, 13, 421-428. https://doi.org/10.3892/etm.2017.4020
MLA
Liu, X., Wang, J., Wang, H., Zhou, C., Yu, Q., Yin, L., Wu, W., Xia, S., Shao, Q."Cell penetrable‑mouse forkhead box P3 suppresses type 1 T helper cell‑mediated immunity in a murine model of delayed‑type hypersensitivity". Experimental and Therapeutic Medicine 13.2 (2017): 421-428.
Chicago
Liu, X., Wang, J., Wang, H., Zhou, C., Yu, Q., Yin, L., Wu, W., Xia, S., Shao, Q."Cell penetrable‑mouse forkhead box P3 suppresses type 1 T helper cell‑mediated immunity in a murine model of delayed‑type hypersensitivity". Experimental and Therapeutic Medicine 13, no. 2 (2017): 421-428. https://doi.org/10.3892/etm.2017.4020