Open Access

Long non‑coding RNA ASncmtRNA‑2 is upregulated in diabetic kidneys and high glucose‑treated mesangial cells

  • Authors:
    • Yan Gao
    • Zhao‑Yu Chen
    • Yan Wang
    • Yan Liu
    • Jian‑Xia Ma
    • Yu‑Kun Li
  • View Affiliations

  • Published online on: January 5, 2017     https://doi.org/10.3892/etm.2017.4027
  • Pages: 581-587
  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Diabetic nephropathy (DN) is one of the most frequent complications associated with type I and II diabetes mellitus. Kidneys from patients with DN are characterized by mesangial matrix expansion and increased thickness of the glomerular basement membrane, which are induced by reactive oxygen species (ROS) production. Previous studies have been conducted to investigate this; however, the detailed mechanism of DN progression remains to be elucidated. The present study evaluated the expression of antisense mitochondrial non‑coding RNA‑2 (ASncmtRNA‑2) in an experimental DN model and cultured human mesangial cells. When mice that exhibited genetic type II diabetes developed DN, ASncmtRNA‑2 expression was significantly increased (P=0.017) and was positively correlated with pro‑fibrotic factor transforming growth factor β1 (TGFβ1) expression and its downstream gene, fibronectin. Inhibition of ROS through administration of the nitric oxide synthase inhibitor, NG‑nitro‑L‑Arginine methylester (L‑NAME), significantly reduced (P=0.022) the upregulation of ASncmtRNA‑2 in DN. In cultured human renal mesangial cells (HRMCs), ASncmtRNA‑2 was upregulated by high glucose stimuli in a time‑dependent manner. Glucose‑induced upregulation of ASncmtRNA‑2 was also reduced by co‑incubation of HRMCs with L‑NAME. Notably, specific short hairpin RNA against ASncmtRNA‑2 significantly downregulated the expression of TGFβ1 in HRMCs. The present study suggests that ASncmtRNA‑2 is upregulated by ROS and may promote glomerular fibrosis in DN via positively regulating the expression of pro‑fibrotic factors. These findings may provide novel potential therapeutic and preventative treatments for DN.

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February-2017
Volume 13 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Gao Y, Chen ZY, Wang Y, Liu Y, Ma JX and Li YK: Long non‑coding RNA ASncmtRNA‑2 is upregulated in diabetic kidneys and high glucose‑treated mesangial cells. Exp Ther Med 13: 581-587, 2017.
APA
Gao, Y., Chen, Z., Wang, Y., Liu, Y., Ma, J., & Li, Y. (2017). Long non‑coding RNA ASncmtRNA‑2 is upregulated in diabetic kidneys and high glucose‑treated mesangial cells. Experimental and Therapeutic Medicine, 13, 581-587. https://doi.org/10.3892/etm.2017.4027
MLA
Gao, Y., Chen, Z., Wang, Y., Liu, Y., Ma, J., Li, Y."Long non‑coding RNA ASncmtRNA‑2 is upregulated in diabetic kidneys and high glucose‑treated mesangial cells". Experimental and Therapeutic Medicine 13.2 (2017): 581-587.
Chicago
Gao, Y., Chen, Z., Wang, Y., Liu, Y., Ma, J., Li, Y."Long non‑coding RNA ASncmtRNA‑2 is upregulated in diabetic kidneys and high glucose‑treated mesangial cells". Experimental and Therapeutic Medicine 13, no. 2 (2017): 581-587. https://doi.org/10.3892/etm.2017.4027