MicroRNA‑200c suppresses cell growth and metastasis by targeting Bmi‑1 and E2F3 in renal cancer cells

Qiu,Mingning; Liang,Ziji; Chen,Lieqian; Tan,Guobin; Liu,Lei; Wang,Kangning; Chen,Hege; Liu,Jianjun

doi:10.3892/etm.2017.4147

MicroRNA‑200c suppresses cell growth and metastasis by targeting Bmi‑1 and E2F3 in renal cancer cells

Authors:
- Mingning Qiu
- Ziji Liang
- Lieqian Chen
- Guobin Tan
- Lei Liu
- Kangning Wang
- Hege Chen
- Jianjun Liu
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Affiliations: Laboratory of Urology, Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China
Published online on: February 21, 2017 https://doi.org/10.3892/etm.2017.4147
Pages: 1329-1336
Copyright: © Qiu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to evaluate the functions of miR‑200c in the regulation of tumor growth and metastasis in renal cancer cells, and to investigate the underlying mechanisms. In this study, miR‑200c was up- and downregulated in two renal cancer cell lines, namely ACHN and A498, and the proliferation, colony formation, migration and invasion of the cells were measured. The expression levels of various mRNAs and proteins were then analyzed using reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. It was found that miR‑200c suppressed proliferation, migration and invasion of the renal cancer cells and, conversely, the inhibition of endogenous miR‑200c resulted in increased cell proliferation and metastasis. Furthermore, a luciferase reporter assay revealed that miR‑200c directly targeted the 3' untranslated regions of the oncogenes B‑cell‑specific Moloney murine leukemia virus insertion site 1 (Bmi‑1) and E2F transcription factor 3 (E2F3) mRNAs, reduced the expression of Bmi‑1 and E2F3 and regulated the expression of downstream genes, including E‑cadherin, N‑cadherin, vimentin, p14 and p16. These results indicate a tumor suppressor role for miR‑200c in renal cancer cells via the direct targeting of Bmi‑1 and E2F3.

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