Open Access

Mediation of insulin growth factor-1 in Alzheimer's disease and the mechanism of PRNP genetic expression and the PI3K/Akt signaling pathway

  • Authors:
    • Guohong Jiang
    • Changming Wang
    • Jun Zhang
    • Haijun Liu
  • View Affiliations

  • Published online on: April 10, 2017     https://doi.org/10.3892/etm.2017.4320
  • Pages: 2763-2766
  • Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the study was to examine the mediation of insulin growth factor-1 (IGF-1) in Alzheimer's disease (AD), as well as the underlying mechanism of the PRNP genetic expression and PI3K/Akt signaling pathway. The Aβ25-35‑incubated rat adrenal pheochromocytoma cell (PC12) in vitro was established, constituting the AD model. Different doses (0, 20, 40 and 80 ng/ml) of IGF-1 were used in PC12 cells and the level of PRNP mRNA was tested after 24 h using the quantitative PCR method and the level of APP protein was assessed using western blot analysis. PC12 cells were divided into the control group (PC12 cells without Aβ25-35 treatment), model group (PC12 cells with Aβ25-35 treatment), IGF-1 80 ng/ml group, IGF-1 80 ng/ml+PI3K inhibitor LY294002 25 µmol/l group, and IGF-1 80 ng/ml+LY294002 50 µmol/l group, whose PRNP mRNA level and Akt, pAkt and APP protein level were tested 24 h later. As the dose of IGF-1 increases, the expression levels of PRNP mRNA and APP protein were more highly expressed. The difference between them was significant (P<0.05). In addition, regarding Akt protein, the expression levels of PRNP mRNA, APP protein and pAkt protein in the IGF-1 groups were significantly higher than those in the control and model groups. With the LY concentration increasing, the levels of expression of the three substances gradually decreased significantly (P<0.05). In conclusion, IGF-I can mediate the expression of the PRNP gene and APP protein through the PI3K/Akt signaling pathway, in a rat model.

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June-2017
Volume 13 Issue 6

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Spandidos Publications style
Jiang G, Wang C, Zhang J and Liu H: Mediation of insulin growth factor-1 in Alzheimer's disease and the mechanism of PRNP genetic expression and the PI3K/Akt signaling pathway. Exp Ther Med 13: 2763-2766, 2017.
APA
Jiang, G., Wang, C., Zhang, J., & Liu, H. (2017). Mediation of insulin growth factor-1 in Alzheimer's disease and the mechanism of PRNP genetic expression and the PI3K/Akt signaling pathway. Experimental and Therapeutic Medicine, 13, 2763-2766. https://doi.org/10.3892/etm.2017.4320
MLA
Jiang, G., Wang, C., Zhang, J., Liu, H."Mediation of insulin growth factor-1 in Alzheimer's disease and the mechanism of PRNP genetic expression and the PI3K/Akt signaling pathway". Experimental and Therapeutic Medicine 13.6 (2017): 2763-2766.
Chicago
Jiang, G., Wang, C., Zhang, J., Liu, H."Mediation of insulin growth factor-1 in Alzheimer's disease and the mechanism of PRNP genetic expression and the PI3K/Akt signaling pathway". Experimental and Therapeutic Medicine 13, no. 6 (2017): 2763-2766. https://doi.org/10.3892/etm.2017.4320