Open Access

Impact of Botox-A SNAP-25 protein expression and the mechanism of inhibitory neurotransmitter imbalance in chronic sciatic nerve pain rat model

  • Authors:
    • Xu‑Dong Ding
    • Wei Wang
    • Zhi‑Gang Ding
    • Yan‑Ping Liu
    • Jing Zhong
    • Hua‑Xian Chen
  • View Affiliations

  • Published online on: April 18, 2017     https://doi.org/10.3892/etm.2017.4351
  • Pages: 2783-2786
  • Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The Botox-A impact on the expression of SNAP-25 protein in rat chronic sciatic nerve pain model was assessed and the mechanism of inhibitory neurotransmitter imbalance was studied. A chronic constriction injury (CCI) model consisted of 30 healthy male rats. The rats were randomly divided into the sham‑operated group, CCI group and BoNT/A intervention group, and during 1, 7 and 14 days we conducted mechanical withdrawal threshold (MWT) test and thermal withdrawal latency (TWL) test before and after operation. After 14 days, the animals were sacrificed. SNAP‑25 protein expression level, mRNA subunit NR2B within excitatory neurotransmitter glutamate GLT and protein expression level, as well as GAT mRNA, the inhibitory GABA neurotransmitter transporter and protein expression level were studied by RT‑polymerase chain reaction and western blot analysis. The difference between MWT and TWL at each point in time before and after operation showed no statistical significance (P>0.05) in the sham‑operated group. For the CCI group at each time point, MWT and TWL were obviously lower than the sham‑operated group and the difference was statistically significant (P<0.05) while the internal difference at each time point showed no statistical significance (P>0.05). The expression level of protein of SNAP‑25 and NR2B mRNA in the CCI group was clearly higher than sham‑operated group. Additionally, the expression level of GAT‑1 mRNA and protein in CCI group was apparently lower than the sham‑operated group. In conclusion, Botox‑A helped reduce SNAP-25 within rat chronic sciatic nerve pain model thereby relieving pain.

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June-2017
Volume 13 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Ding XD, Wang W, Ding ZG, Liu YP, Zhong J and Chen HX: Impact of Botox-A SNAP-25 protein expression and the mechanism of inhibitory neurotransmitter imbalance in chronic sciatic nerve pain rat model. Exp Ther Med 13: 2783-2786, 2017.
APA
Ding, X., Wang, W., Ding, Z., Liu, Y., Zhong, J., & Chen, H. (2017). Impact of Botox-A SNAP-25 protein expression and the mechanism of inhibitory neurotransmitter imbalance in chronic sciatic nerve pain rat model. Experimental and Therapeutic Medicine, 13, 2783-2786. https://doi.org/10.3892/etm.2017.4351
MLA
Ding, X., Wang, W., Ding, Z., Liu, Y., Zhong, J., Chen, H."Impact of Botox-A SNAP-25 protein expression and the mechanism of inhibitory neurotransmitter imbalance in chronic sciatic nerve pain rat model". Experimental and Therapeutic Medicine 13.6 (2017): 2783-2786.
Chicago
Ding, X., Wang, W., Ding, Z., Liu, Y., Zhong, J., Chen, H."Impact of Botox-A SNAP-25 protein expression and the mechanism of inhibitory neurotransmitter imbalance in chronic sciatic nerve pain rat model". Experimental and Therapeutic Medicine 13, no. 6 (2017): 2783-2786. https://doi.org/10.3892/etm.2017.4351