Open Access

Biocompatibility of polypropylene mesh scaffold with adipose-derived stem cells

  • Authors:
    • Hui Cheng
    • Yanling Zhang
    • Bei Zhang
    • Jie Cheng
    • Weiqi Wang
    • Xin Tang
    • Peng Teng
    • Yanyu Li
  • View Affiliations

  • Published online on: April 13, 2017     https://doi.org/10.3892/etm.2017.4338
  • Pages: 2922-2926
  • Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In this study, we investigated the rejection of the synthetic patch and human tissues in the host. We observed the growth of adipose-derived stem cells (ADSCs) cultured with polypropylene mesh in vitro. The results of flow cytometry showed that the expression of CD44, CD73, CD90, CD45, CD14 and CD34 was 98.54, 95.32, 98.49, 1.21, 3.01 and 2.14%, respectively. ADSCs were isolated from rabbit subcutaneous adipose tissue after collagenase digestion, filtration and centrifugation. The ADSCs of passage 3 were seeded onto the polypropylene mesh scaffolds. New Zealand White female breeder rabbits were implanted with polypropylene mesh, ADSC-fixed polypropylene mesh in the abdomen. After 4 weeks, adhesion was performed and the erosion of the mesh was evaluated. It was found that polypropylene mesh, ADSC-fixed polypropylene mesh all had different degrees of corrosion, and adhesion, but polypropylene mesh was more corroded. ADSC-fixed polypropylene mesh induced a milder chronic inflammation response compared with polypropylene, had significantly lower scores for inflammation (t=11.083), and had significantly higher scores for neovascularization (t=14.362) and fibroblastic proliferation (t=15.979). The relative amount of VEGF mRNA was significantly lower for ADSC-fixed polypropylene compared with the other polypropylene meshes (t=94.6). In conclusion, polypropylene mesh scaffold with ADSCs exhibit excellent cellular compatibility and are promising in clinical practice.

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June-2017
Volume 13 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Cheng H, Zhang Y, Zhang B, Cheng J, Wang W, Tang X, Teng P and Li Y: Biocompatibility of polypropylene mesh scaffold with adipose-derived stem cells. Exp Ther Med 13: 2922-2926, 2017
APA
Cheng, H., Zhang, Y., Zhang, B., Cheng, J., Wang, W., Tang, X. ... Li, Y. (2017). Biocompatibility of polypropylene mesh scaffold with adipose-derived stem cells. Experimental and Therapeutic Medicine, 13, 2922-2926. https://doi.org/10.3892/etm.2017.4338
MLA
Cheng, H., Zhang, Y., Zhang, B., Cheng, J., Wang, W., Tang, X., Teng, P., Li, Y."Biocompatibility of polypropylene mesh scaffold with adipose-derived stem cells". Experimental and Therapeutic Medicine 13.6 (2017): 2922-2926.
Chicago
Cheng, H., Zhang, Y., Zhang, B., Cheng, J., Wang, W., Tang, X., Teng, P., Li, Y."Biocompatibility of polypropylene mesh scaffold with adipose-derived stem cells". Experimental and Therapeutic Medicine 13, no. 6 (2017): 2922-2926. https://doi.org/10.3892/etm.2017.4338