Open Access

Diazoxide protects rat vascular endothelial cells against hypoxia and cold-induced damage

  • Authors:
    • Lian‑Cheng Zhang
    • Zhao Huang
    • Pei‑Bing Li
    • Hong‑Jing Nie
    • Bing‑Nan Deng
    • Rui‑Feng Duan
    • Zhong‑Hai Xiao
    • Hui Peng
    • Hong Feng
    • Wei Liu
  • View Affiliations

  • Published online on: May 5, 2017     https://doi.org/10.3892/etm.2017.4437
  • Pages: 3257-3266
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to examine the effects of hypoxia and cold on vascular endothelial cells (VECs), as well as the protective ability of novel VECs‑protective drugs against these injuries. A rat model simulating exposure to hypoxia and cold at high altitude environments was established. Based on these animal experiments, rat aortic VECs were established as injury models and exposed to hypoxia and/or adrenaline (ADR) in vitro. The results revealed that hypoxia significantly altered the levels of nitric oxide and vascular endothelial growth factor, while the cold temperature significantly increased the release of ADR and noradrenaline. Exposure to hypoxia combined with cold temperature significantly affected all these indices. In vitro experiments demonstrated that hypoxia, ADR (which was used to simulate cold in the animal experiments) and the combination of the two factors resulted in damage to the VECs and endothelial dysfunction. In addition, the results also showed that diazoxide, a highly selective mitoKATP opener, protected VECs against these injuries. In conclusion, hypoxia and cold temperature induced endothelial cell dysfunction and endocrine disorders, respectively. Improving endothelial function using diazoxide may be an effective therapeutic strategy in patients with altitude‑associated disorders. However, the potential for clinical application requires further study.

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June-2017
Volume 13 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Zhang LC, Huang Z, Li PB, Nie HJ, Deng BN, Duan RF, Xiao ZH, Peng H, Feng H, Liu W, Liu W, et al: Diazoxide protects rat vascular endothelial cells against hypoxia and cold-induced damage. Exp Ther Med 13: 3257-3266, 2017
APA
Zhang, L., Huang, Z., Li, P., Nie, H., Deng, B., Duan, R. ... Liu, W. (2017). Diazoxide protects rat vascular endothelial cells against hypoxia and cold-induced damage. Experimental and Therapeutic Medicine, 13, 3257-3266. https://doi.org/10.3892/etm.2017.4437
MLA
Zhang, L., Huang, Z., Li, P., Nie, H., Deng, B., Duan, R., Xiao, Z., Peng, H., Feng, H., Liu, W."Diazoxide protects rat vascular endothelial cells against hypoxia and cold-induced damage". Experimental and Therapeutic Medicine 13.6 (2017): 3257-3266.
Chicago
Zhang, L., Huang, Z., Li, P., Nie, H., Deng, B., Duan, R., Xiao, Z., Peng, H., Feng, H., Liu, W."Diazoxide protects rat vascular endothelial cells against hypoxia and cold-induced damage". Experimental and Therapeutic Medicine 13, no. 6 (2017): 3257-3266. https://doi.org/10.3892/etm.2017.4437