Open Access

Establishment and evaluation of a reversible two‑kidney, one‑clip renovascular hypertensive rat model

  • Authors:
    • Li‑Qiang Li
    • Jian Zhang
    • Rong Wang
    • Jian‑Xin Li
    • Yong‑Quan Gu
  • View Affiliations

  • Published online on: April 26, 2017     https://doi.org/10.3892/etm.2017.4386
  • Pages: 3291-3296
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to establish and evaluate a novel and reversible two‑kidney, one‑clip renovascular hypertensive rat model with a titanium vascular clip. A total of 40 male Sprague‑Dawley rats were evenly and randomly divided into a sham‑operated group, and 3, 7, 12 and 28D groups (namely removing the vascular clip in the renovascular hypertensive model after 3, 7, 12 and 28 days, respectively). The systolic blood pressure (SBP) and plasma renin activity (PRA) were measured, and color duplex imaging was conducted before placing the clips, as well as before and after removing them. After placing the vascular clips, SBP and PRA in the 3, 7, 12 and 28D groups were significantly increased (SBP: Sham‑operated vs. 3D groups, P=0.020; 3 vs. 7D groups, P=0.008; 7 vs. 28D groups, P=0.019; 12 vs. 28D groups, P=0.039, and between other groups P<0.001. PRA: 3 vs. 7D groups, P=0.001; 7 vs. 12D groups, P=0.004; 12 vs. 28D groups, P=0.040, and between other groups, P<0.001). After removing the clips, SBP were significantly reduced in the 3 and 7D groups (P=0.023, 0.040, 0.066 and 0.314 in the 3, 7, 12 and 28D groups, respectively), but were still significantly higher than that before placing clips in the 7, 12 and 28D groups (P=0.067, P=0.005, P<0.001 and P<0.001 in the 3, 7, 12 and 28D groups, respectively). After removing the clips, PRA was significantly reduced in each group (P<0.001, P<0.001, P=0.012 and P=0.049 in 3, 7, 12 and 28D groups, respectively), but still higher than that before placing the clips (P<0.001, P=0.001, P=0.001 and P=0.003 in 3, 7, 12 and 28D groups, respectively). Vascular imaging also indicates this model has a reversible property. In conclusion, a reversible renovascular hypertension rat model is feasible, and provides a basis for research on clinical ischemic nephropathy and renal artery revascularization.

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June-2017
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Spandidos Publications style
Li LQ, Zhang J, Wang R, Li JX and Gu YQ: Establishment and evaluation of a reversible two‑kidney, one‑clip renovascular hypertensive rat model. Exp Ther Med 13: 3291-3296, 2017
APA
Li, L., Zhang, J., Wang, R., Li, J., & Gu, Y. (2017). Establishment and evaluation of a reversible two‑kidney, one‑clip renovascular hypertensive rat model. Experimental and Therapeutic Medicine, 13, 3291-3296. https://doi.org/10.3892/etm.2017.4386
MLA
Li, L., Zhang, J., Wang, R., Li, J., Gu, Y."Establishment and evaluation of a reversible two‑kidney, one‑clip renovascular hypertensive rat model". Experimental and Therapeutic Medicine 13.6 (2017): 3291-3296.
Chicago
Li, L., Zhang, J., Wang, R., Li, J., Gu, Y."Establishment and evaluation of a reversible two‑kidney, one‑clip renovascular hypertensive rat model". Experimental and Therapeutic Medicine 13, no. 6 (2017): 3291-3296. https://doi.org/10.3892/etm.2017.4386