Open Access

Effects of 1,25‑dihydroxyvitamin D3 on the local bone renin‑angiotensin system in a murine model of glucocorticoid‑induced osteoporosis

  • Authors:
    • Lin Shen
    • Chen Ma
    • Bo Shuai
    • Yanping Yang
  • View Affiliations

  • Published online on: April 28, 2017     https://doi.org/10.3892/etm.2017.4404
  • Pages: 3297-3304
  • Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Active vitamin D is closely related to the circulating renin-angiotensin system (RAS) in experimental animal models and humans; however, corresponding local bone data remain limited. The present study examined whether 1,25‑dihydroxyvitamin D3 supplementation altered local bone RAS elements in a murine model of glucocorticoid‑induced osteoporosis (GIOP). A total of 36 8‑week‑old mice were randomized into three equal‑sized groups: The sham, GIOP and 1,25‑dihydroxyvitamin D3 treatment groups. After 12 weeks, the cancellous bone microstructure of the third lumbar vertebra and left femur from the mice from each group were examined using micro‑computed tomography. To access the impact of glucocorticoid use, the effect of 1,25‑dihydroxyvitamin D3 on cancellous bone microstructure, the expression of bone turnover markers, circulation and expression of the main RAS components was assessed. Results demonstrated that bone volume fraction, trabecular number and trabecular thickness of the treatment and sham groups were significantly higher than the GIOP group (P<0.05). Furthermore, the structure model index, trabecular separation and bone surface to bone volume ratio of the sham and treatment groups were significantly reduced compared with the GIOP group (P<0.05). All assessed parameters exhibited no significant differences between the treatment and sham groups. mRNA expression levels of local bone angiotensin type 1 and 2 receptors and receptor activator of nuclear factor‑κB ligand were significantly lower in the treatment group than in the GIOP group (P<0.05); however, there were no significant differences in circulating protein levels between the groups (P>0.05). In conclusion, 1,25‑dihydroxyvitamin D3 may modulate bone metabolism by downregulating the local bone RAS in mice with GIOP.

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June-2017
Volume 13 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Shen L, Ma C, Shuai B and Yang Y: Effects of 1,25‑dihydroxyvitamin D3 on the local bone renin‑angiotensin system in a murine model of glucocorticoid‑induced osteoporosis. Exp Ther Med 13: 3297-3304, 2017
APA
Shen, L., Ma, C., Shuai, B., & Yang, Y. (2017). Effects of 1,25‑dihydroxyvitamin D3 on the local bone renin‑angiotensin system in a murine model of glucocorticoid‑induced osteoporosis. Experimental and Therapeutic Medicine, 13, 3297-3304. https://doi.org/10.3892/etm.2017.4404
MLA
Shen, L., Ma, C., Shuai, B., Yang, Y."Effects of 1,25‑dihydroxyvitamin D3 on the local bone renin‑angiotensin system in a murine model of glucocorticoid‑induced osteoporosis". Experimental and Therapeutic Medicine 13.6 (2017): 3297-3304.
Chicago
Shen, L., Ma, C., Shuai, B., Yang, Y."Effects of 1,25‑dihydroxyvitamin D3 on the local bone renin‑angiotensin system in a murine model of glucocorticoid‑induced osteoporosis". Experimental and Therapeutic Medicine 13, no. 6 (2017): 3297-3304. https://doi.org/10.3892/etm.2017.4404