Open Access

Evaluation of the shape, viability, stemness and osteogenic differentiation of cell spheroids formed from human gingiva‑derived stem cells and osteoprecursor cells

  • Authors:
    • Sung‑Il Lee
    • Youngkyung Ko
    • Jun‑Beom Park
  • View Affiliations

  • Published online on: April 26, 2017     https://doi.org/10.3892/etm.2017.4388
  • Pages: 3467-3473
  • Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study was performed to create stem cell spheroids from human gingiva‑derived stem cells and osteoprecursor cells and to evaluate the maintenance of the stemness, the viability and osteogenic differentiation of the cell spheroids. Gingiva‑derived stem cells were isolated, and a total of 6x105 stem cells and osteoprecursor cells were seeded into concave micromolds at various ratios. Gingiva‑derived stem cells and/or osteoprecursor cells formed spheroids in concave microwells. The spheroids demonstrated a smaller diameter when the number of osteoprecursor cells seeded was lower. The majority of cells in the spheroids were identified to be live cells and the cell spheroids preserved viability throughout the experimental period. The cell spheroids, which contained stem cells, were positive for stem‑cell markers. Cell spheroids in concave microwells demonstrated a statistically significant increase in alkaline phosphatase activity as time progressed (P<0.05). A statistically significant difference in phosphatase activity was observed in the stem cell alone group when compared with the osteoprecursor cell group at day 5 (P<0.05). Mineralized extracellular deposits were observed in each group after Alizarin Red S staining. Within the limits of the present study, cell spheroids from gingival cells and osteoprecursor cells maintained shape, viability, stemness and osteogenic differentiation potential.

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June-2017
Volume 13 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Lee SI, Ko Y and Park JB: Evaluation of the shape, viability, stemness and osteogenic differentiation of cell spheroids formed from human gingiva‑derived stem cells and osteoprecursor cells. Exp Ther Med 13: 3467-3473, 2017.
APA
Lee, S., Ko, Y., & Park, J. (2017). Evaluation of the shape, viability, stemness and osteogenic differentiation of cell spheroids formed from human gingiva‑derived stem cells and osteoprecursor cells. Experimental and Therapeutic Medicine, 13, 3467-3473. https://doi.org/10.3892/etm.2017.4388
MLA
Lee, S., Ko, Y., Park, J."Evaluation of the shape, viability, stemness and osteogenic differentiation of cell spheroids formed from human gingiva‑derived stem cells and osteoprecursor cells". Experimental and Therapeutic Medicine 13.6 (2017): 3467-3473.
Chicago
Lee, S., Ko, Y., Park, J."Evaluation of the shape, viability, stemness and osteogenic differentiation of cell spheroids formed from human gingiva‑derived stem cells and osteoprecursor cells". Experimental and Therapeutic Medicine 13, no. 6 (2017): 3467-3473. https://doi.org/10.3892/etm.2017.4388