Rutin suppresses high glucose-induced ACTA2 and p38 protein expression in diabetic nephropathy

Han,Chun‑Shan; Liu,Kai; Zhang,Ning; Li,Shi‑Wen; Gao,Hai‑Cheng

doi:10.3892/etm.2017.4509

Rutin suppresses high glucose-induced ACTA2 and p38 protein expression in diabetic nephropathy

Authors:
- Chun‑Shan Han
- Kai Liu
- Ning Zhang
- Shi‑Wen Li
- Hai‑Cheng Gao
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Affiliations: Department of Chest Surgery, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Department of Pathology and Pathophysiology, Chengde Medical College, Chengde, Hebei 300000, P.R. China, Department of Clinical Pharmacy, Jilin University School of Pharmaceutical Sciences, Changchun, Jilin 130021, P.R. China
Published online on: May 23, 2017 https://doi.org/10.3892/etm.2017.4509
Pages: 181-186
Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study investigated the effect of rutin on high glucose-induced actin, α2, smooth muscle, aorta (ACTA2) and p38 protein expression in diabetic nephropathy (DN). Human mesangial cells were divided into a control group, high glucose‑induced mesangial cell group, high glucose + captopril group, and high glucose + rutin group (low, middle and high doses of rutin). Cell viability, adenosine 5'‑triphosphate (ATP) content, cell cycle, and ACTA2 and p38 protein expression were examined using MTT assay, ATP assay kit, flow cytometry and immunofluorescence staining in cultured human mesangial cells, respectively. Cell viability, ATP content, and ACTA2 and p38 expression increased significantly in high glucose‑induced mesangial cells (P<0.05). However, at concentrations of 0.2, 0.4 and 0.8 µmol/l rutin was able to inhibit high glucose‑induced human mesangial cell viability, ATP content, and ACTA2 and p38 expression and improve the cell cycle progression of mesangial cells. In conclusion, ACTA2 and p38 proteins may have important roles in DN. Rutin may inhibit the expression of ACTA2 and p38 and may be utilized in the prevention and treatment of DN.

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