Role of transient receptor potential channel 6 in the odontogenic differentiation of human dental pulp cells

Yang,Xiaoting; Song,Zhi; Chen,Lingling; Wang,Runfu; Huang,Shuheng; Qin,Wei; Guo,Jia; Lin,Zhengmei

doi:10.3892/etm.2017.4471

Role of transient receptor potential channel 6 in the odontogenic differentiation of human dental pulp cells

Authors:
- Xiaoting Yang
- Zhi Song
- Lingling Chen
- Runfu Wang
- Shuheng Huang
- Wei Qin
- Jia Guo
- Zhengmei Lin
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Affiliations: Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat‑sen University, Guangzhou, Guangdong 510055, P.R. China
Published online on: May 18, 2017 https://doi.org/10.3892/etm.2017.4471
Pages: 73-78
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Pulp capping is a restorative technique employed in an attempt to maintain pulpal vitality and generate reparative dentin. Ca2+ released from capping materials is suggested to promote reparative dentin formation. Transient receptor potential channel 6 (TRPC6) is a receptor‑operated Ca2+ channel that serves an important role in Ca2+ influx in the majority of non‑excitable cells, and influences the calcium signaling and cell respond. Therefore, the purpose of the present study was to gain an insight into the role of TRPC6 in the odontoblastic differentiation of human dental pulp cells (HDPCs). Human dental pulp tissues and HDPCs were obtained from healthy third molars. By immunohistochemical staining, TRPC6 was observed to be highly expressed in the dental pulp tissue, particularly in the odontoblast layer. In addition, the protein level of TRPC6 was increased in a time‑dependent manner during odontogenic differentiation of HDPCs. Downregulation of TRPC6 by a lentivirus vector containing TRPC6 shRNA inhibited the process of odontogenic differentiation in HDPCs. In conclusion, the current data demonstrated that TRPC6 served a significant role in the odontogenic differentiation of HDPCs, suggesting it may be a promising therapeutic target in regenerative endodontics.

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