Pien Tze Huang alleviates 5‑fluorouracil‑induced intestinal mucositis in CT‑26 tumor‑bearing mice

  • Authors:
    • Caixuan Fu
    • Jianfeng Chu
    • Aling Shen
    • Liya Liu
    • Hongwei Chen
    • Jiumao Lin
    • Thomas J. Sferra
    • Youqin Chen
    • Jun Peng
  • View Affiliations

  • Published online on: July 10, 2017     https://doi.org/10.3892/etm.2017.4755
  • Pages: 2291-2297
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Abstract

Chemotherapeutic agents, including 5‑fluorouracil (5‑FU), frequently cause intestinal mucositis as a side effect, leading to life quality reduction in colorectal cancer (CRC) patients and interruption of CRC treatment. Traditional Chinese medicines (TCMs) have recently received attention due to their relatively few adverse effects. Pien Tze Huang (PZH), a well‑known TCM formulation first documented to have been prescribed >450 years ago, has been demonstrated to be clinically effective in treating various types of cancer including CRC. Although previous studies by our group reported that PZH possesses a variety of anti‑cancer activities via multiple mechanisms, it has remained elusive whether it is able to reduce intestinal mucositis induced by 5‑FU. The present study evaluated the effect of PZH on 5‑FU‑induced intestinal mucositis in CT‑26 tumor‑bearing xenograft mice and investigated the possible molecular mechanism. The results indicated that administration of PZH effectively alleviated the severity of 5‑FU‑induced diarrhea and morphological intestinal damages, but had no significant effect on body weight loss. In addition, a terminal deoxynucleotidyl transferase dUTP nick end labeling assay revealed that PZH treatment significantly inhibited cell apoptosis in the intestinal crypt. Furthermore, immunohistochemical staining showed that PZH treatment reduced the protein expression of pro‑apoptotic B‑cell lymphoma 2 (Bcl‑2)‑associated X protein but enhanced that of anti‑apoptotic Bcl‑2 in the intestinal crypt. Taken together, the results of the present study suggested that PZH effectively attenuates 5‑FU‑induced intestinal mucositis, which is in part associated with its inhibitory effect on cell apoptosis in the intestinal crypt.

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September-2017
Volume 14 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Fu C, Chu J, Shen A, Liu L, Chen H, Lin J, Sferra TJ, Chen Y and Peng J: Pien Tze Huang alleviates 5‑fluorouracil‑induced intestinal mucositis in CT‑26 tumor‑bearing mice. Exp Ther Med 14: 2291-2297, 2017
APA
Fu, C., Chu, J., Shen, A., Liu, L., Chen, H., Lin, J. ... Peng, J. (2017). Pien Tze Huang alleviates 5‑fluorouracil‑induced intestinal mucositis in CT‑26 tumor‑bearing mice. Experimental and Therapeutic Medicine, 14, 2291-2297. https://doi.org/10.3892/etm.2017.4755
MLA
Fu, C., Chu, J., Shen, A., Liu, L., Chen, H., Lin, J., Sferra, T. J., Chen, Y., Peng, J."Pien Tze Huang alleviates 5‑fluorouracil‑induced intestinal mucositis in CT‑26 tumor‑bearing mice". Experimental and Therapeutic Medicine 14.3 (2017): 2291-2297.
Chicago
Fu, C., Chu, J., Shen, A., Liu, L., Chen, H., Lin, J., Sferra, T. J., Chen, Y., Peng, J."Pien Tze Huang alleviates 5‑fluorouracil‑induced intestinal mucositis in CT‑26 tumor‑bearing mice". Experimental and Therapeutic Medicine 14, no. 3 (2017): 2291-2297. https://doi.org/10.3892/etm.2017.4755