Open Access

Oxidized low‑density lipoprotein‑induced p62/SQSTM1 accumulation in THP‑1‑derived macrophages promotes IL‑18 secretion and cell death

  • Authors:
    • Haofeng Ning
    • Dan Liu
    • Xiaochen Yu
    • Xiuru Guan
  • View Affiliations

  • Published online on: September 29, 2017     https://doi.org/10.3892/etm.2017.5221
  • Pages:5417-5423
  • Copyright: © Ning et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Macrophage autophagy has a protective role in the development of atherosclerosis; however, it turns dysfunctional in advanced lesions with an increase in p62/sequestosome‑1 protein. Little is known about the role and significance of p62 accumulation in atherosclerosis. The present study investigated the association between p62 expression and the process of foam cell formation. Foam cell models were established through incubation of THP‑1‑derived macrophages with oxidized low‑density lipoprotein, and the process of foam cell formation was detected by Oil red O staining. Furthermore, the dynamic change of p62 expression was detected by western blotting and quantitative polymerase chain reaction. Additionally, using gene silencing techniques, the roles of p62 in foam cells were investigated with ELISA, MTT and flow cytometry. The results indicated that besides serving as a marker of autophagy deficiency, the p62 protein could also mediate inflammation and cytotoxicity in advanced foam cells. Additionally, the implication of p62 in autophagy inhibition and foam cell formation makes it a key atherogenic factor under autophagy‑deficient conditions.

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Journal Cover

December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

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APA
Ning, H., Liu, D., Yu, X., & Guan, X. (2017). Oxidized low‑density lipoprotein‑induced p62/SQSTM1 accumulation in THP‑1‑derived macrophages promotes IL‑18 secretion and cell death. Experimental and Therapeutic Medicine, 14, 5417-5423. https://doi.org/10.3892/etm.2017.5221
MLA
Ning, H., Liu, D., Yu, X., Guan, X."Oxidized low‑density lipoprotein‑induced p62/SQSTM1 accumulation in THP‑1‑derived macrophages promotes IL‑18 secretion and cell death". Experimental and Therapeutic Medicine 14.6 (2017): 5417-5423.
Chicago
Ning, H., Liu, D., Yu, X., Guan, X."Oxidized low‑density lipoprotein‑induced p62/SQSTM1 accumulation in THP‑1‑derived macrophages promotes IL‑18 secretion and cell death". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5417-5423. https://doi.org/10.3892/etm.2017.5221