Open Access

BMP‑7 accelerates the differentiation of rabbit mesenchymal stem cells into cartilage through the Wnt/β‑catenin pathway

  • Authors:
    • Hong‑Dan Fu
    • Hai‑Rui Wang
    • Dai‑He Li
  • View Affiliations

  • Published online on: September 27, 2017     https://doi.org/10.3892/etm.2017.5210
  • Pages:5424-5428
  • Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Mesenchymal stem cells (MSCs) are able to differentiate into adipocytes, chondroblasts or cartilage under different stimulation conditions. Identifying a mechanism that triggers the differentiation of MSCs into cartilage may help the development of novel therapeutic approaches for heterotopic ossification, the pathological formation of lamellar bone in soft tissue outside the skeleton that may lead to debilitating immobility. Bone morphogenetic proteins (BMPs), including BMP‑7, are the most potent growth factors for enhancing bone formation. The current study aimed to understand the potential involvement of the Wnt/β‑catenin signaling pathway in the BMP‑7‑induced growth of rabbit MSCs (rMSCs). Different concentrations of BMP‑7 were applied to cultured rMSCs, and proliferation was evaluated by MTT assay. Changes in the phosphorylation state of glycogen synthase kinase (GSK)‑3β, in addition to the expression levels of alkaline phosphatase, β‑catenin and runt‑related transcription factor 2 were observed by western blot analysis. Following treatment with BMP‑7, the phosphorylation of GSK‑3β was stimulated and the expression of β‑catenin, ALP and Runx2 was increased. Furthermore, inhibiting β‑catenin signaling with XAV‑939 suppressed the BMP‑7‑mediated changes. The results indicated that the BMP‑7‑induced differentiation of rMSCs into cartilage was promoted primarily by the Wnt/β‑catenin pathway.

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Journal Cover

December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

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APA
Fu, H., Wang, H., & Li, D. (2017). BMP‑7 accelerates the differentiation of rabbit mesenchymal stem cells into cartilage through the Wnt/β‑catenin pathway. Experimental and Therapeutic Medicine, 14, 5424-5428. https://doi.org/10.3892/etm.2017.5210
MLA
Fu, H., Wang, H., Li, D."BMP‑7 accelerates the differentiation of rabbit mesenchymal stem cells into cartilage through the Wnt/β‑catenin pathway". Experimental and Therapeutic Medicine 14.6 (2017): 5424-5428.
Chicago
Fu, H., Wang, H., Li, D."BMP‑7 accelerates the differentiation of rabbit mesenchymal stem cells into cartilage through the Wnt/β‑catenin pathway". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5424-5428. https://doi.org/10.3892/etm.2017.5210