Open Access

Identification of microRNAs in human circulating monocytes of postmenopausal osteoporotic Mexican‑Mestizo women: A pilot study

  • Authors:
    • Rogelio F. Jiménez‑Ortega
    • Eric G. Ramírez‑Salazar
    • Alma Y. Parra‑Torres
    • Said A. Muñoz‑Montero
    • Claudia Rangel‑Escareňo
    • Ivan Salido‑Guadarrama
    • Mauricio Rodriguez‑Dorantes
    • Manuel Quiterio
    • Jorge Salmerón
    • Rafael Velázquez‑Cruz
  • View Affiliations

  • Published online on: October 3, 2017     https://doi.org/10.3892/etm.2017.5260
  • Pages:5464-5472
  • Copyright: © Jiménez‑Ortega et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs or miRs) are a class of short non‑coding RNAs that serve an important regulatory role in living organisms. These molecules are associated with multiple biological processes and are potential biomarkers in multiple diseases. The present study aimed to further identify miRNAs that are differentially expressed in circulating monocytes (CMCs) from postmenopausal Mexican‑Mestizo women. Microarray analyses of monocytes using Affymetrix miRNA 4.0 and Human Genome U133 Plus 2.0 arrays were performed in 6 normal and 6 osteoporotic women, followed by reverse transcription-quantitative polymerase chain reaction (RT‑qPCR) validation. The overexpression of miR‑1270, miR‑548x‑3p and miR‑8084 were detected in the osteoporosis compared with the normal group according to the microarray analysis; miR‑1270, a miRNA with several target genes associated with bone remodeling, was validated by RT‑qPCR. Bioinformatics analysis identified that interferon regulatory factor 8 (IRF8) is the most likely target gene of miR‑1270, which is associated with osteoclastogenesis. Furthermore, the findings of the present study demonstrate that an upregulation of miR‑1270 may reduce the gene expression of IRF8 in CMCs (osteoclast precursors), implicating its potential role in leading to low bone mineral density and contributing to osteoporosis development in postmenopausal women.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Jiménez‑Ortega, R.F., Ramírez‑Salazar, E.G., Parra‑Torres, A.Y., Muñoz‑Montero, S.A., Rangel‑Escareňo, C., Salido‑Guadarrama, I. ... Velázquez‑Cruz, R. (2017). Identification of microRNAs in human circulating monocytes of postmenopausal osteoporotic Mexican‑Mestizo women: A pilot study. Experimental and Therapeutic Medicine, 14, 5464-5472. https://doi.org/10.3892/etm.2017.5260
MLA
Jiménez‑Ortega, R. F., Ramírez‑Salazar, E. G., Parra‑Torres, A. Y., Muñoz‑Montero, S. A., Rangel‑Escareňo, C., Salido‑Guadarrama, I., Rodriguez‑Dorantes, M., Quiterio, M., Salmerón, J., Velázquez‑Cruz, R."Identification of microRNAs in human circulating monocytes of postmenopausal osteoporotic Mexican‑Mestizo women: A pilot study". Experimental and Therapeutic Medicine 14.6 (2017): 5464-5472.
Chicago
Jiménez‑Ortega, R. F., Ramírez‑Salazar, E. G., Parra‑Torres, A. Y., Muñoz‑Montero, S. A., Rangel‑Escareňo, C., Salido‑Guadarrama, I., Rodriguez‑Dorantes, M., Quiterio, M., Salmerón, J., Velázquez‑Cruz, R."Identification of microRNAs in human circulating monocytes of postmenopausal osteoporotic Mexican‑Mestizo women: A pilot study". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5464-5472. https://doi.org/10.3892/etm.2017.5260