Pterostilbene attenuates myocardial ischemia‑reperfusion injury via the phosphatidylinositol 3'‑kinase‑protein kinase B signaling pathway

  • Authors:
    • Lei Hu
    • Na Cai
    • Hui Jia
  • View Affiliations

  • Published online on: October 2, 2017     https://doi.org/10.3892/etm.2017.5246
  • Pages: 5509-5514
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The current study aimed to evaluate the cardioprotective effects of pterostilbene (PTB) on myocardial ischemia‑reperfusion (I/R) injury in rats and identify its possible underlying mechanisms of action. A rat I/R model was established by ligating the left anterior descending coronary artery for 30 min and releasing the ligature to induce reperfusion for 120 min. Serum creatine kinase‑MB (CK‑MB) and lactate dehydrogenase (LDH) levels were measured using CK‑MB and LDH assay kits and myeloperoxidase (MPO) activity in the myocardium was evaluated using an MPO assay kit. Tumor necrosis factor‑α, interleukin (IL)‑6 and IL‑8 levels were assayed using ELISA kits. Cardiomyocyte apoptosis was measured using terminal deoxynucleotidyl transferase dUTP nick end labeling staining. Levels of protein kinase B (Akt) and phosphorylated Akt (p‑Akt) were measured using western blotting. The results demonstrated that treatment with PTB significantly reduced cardiomyocyte apoptosis, significantly increased Bcl‑2 and p‑Akt levels and decreased Bax expression in the hearts of rats subjected to I/R injury. However, the protective effects induced by PTB were attenuated by LY294002, which inhibits Akt activation. The results of the current study suggest that PTB treatment may reduce the I/R injury‑induced apoptosis of cardiomyocytes, which is mediated by the phosphoinositide 3‑kinase/Akt signaling pathway.

Introduction

Myocardial infarction is a major cause of mortality and morbidity (1). Currently the main method of treating myocardial infarction is the re-establishment of blood flow as early as possible to prevent further injury to the myocardium. However, this may cause myocardial ischemia/reperfusion (I/R) injury and there are currently no therapeutic strategies available to treat this (2). Myocardial I/R injury induces a series of pathological changes, including cellular apoptosis, calcium overload and oxidative stress (3,4). Therefore, it is critical to identify novel therapeutic agents to reduce I/R injury in patients with myocardial infarction.

Pterostilbene (PTB), a natural phytoalexin found in blueberries, is a dimethylated analog of resveratrol (5). Previous studies have demonstrated that PTB exhibits various biological roles, including anti-inflammatory, anti-oxidation and anti-apoptotic functions (68). Oxidative stress, apoptosis and inflammation serve critical roles in myocardial I/R injury; thus, PTB is a promising candidate for the treatment of myocardial I/R injury. However, the effects of PTB on myocardial I/R injury remain unclear.

The phosphatidylinositol 3′-kinase-protein kinase B (PI3K/Akt) signaling pathway co-ordinates various intracellular processes, including regulation of cell proliferation and survival (9,10). The PI3K/Akt signaling pathway is a pro-survival signaling pathway that confers protection against myocardial ischemia (11). However, it remains unknown whether PTB confers cardioprotection via activation of the PI3K/Akt signaling pathway. Therefore, the current study aimed to determine whether PTB reduces myocardial I/R injury and if so, identify whether PI3K/Akt signaling is activated by treatment with PTB.

Materials and methods

Animals

A total of 60 adult male Sprague-Dawley rats (250–300 g; 1.5–2 months) were obtained from the Center of Experimental Animals in the Xi'an Jiaotong University (Xi'an, China). Animals were housed in a controlled room with a temperature of 22°C, 33% humidity, a 12 h light/dark cycle and free access to food and water. All animals used in the current study were cared for in accordance with the Guide for the Care and Use of Laboratory Animals published by the National Research Council (US) Committee (12) and all procedures were approved by The Committee of Experimental Animals of the Xi'an Jiaotong University.

Reagents

PTB, the PI3K inhibitor LY294002 and DAPI were all purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). Myocardial myeloperoxidase (MPO; cat. no. A044), creatine kinase-MB (CK-MB; cat. no. H197) and lactate dehydrogenase (LDH; cat. no. A020-1) assay kits were all purchased from Nanjing Jiancheng Bioengineering Research Institute (Nanjing, China). Interleukin-6 (IL-6; cat. no. R6000B) and tumor necrosis factor-α (TNF-α; cat. no. RTA00) ELISA kits were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). IL-8 ELISA kit (cat. no. kt30449) was purchased from MSK Bio (Wuhan, China). Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) kits were purchased from Roche Applied Science (Mannheim, Germany). Antibodies against Akt (cat. no. 4685), p-Akt (Ser473; cat. no. 4058), Bcl-2 (cat. no. 3498), Bax (cat. no. 14796) and β-actin (cat. no. 4970) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The horseradish peroxidase-conjugated immunoglobulin G secondary antibody (cat. no. A0208) was purchased from Beyotime Institute of Biotechnology (Haimen, China).

Myocardial ischemia-reperfusion (MI/R) model and experimental protocol

Sprague-Dawley rats were anesthetized intraperitoneally (i.p.) using sodium pentobarbital (Sigma-Aldrich; Merck KGaA; 40 mg/kg). Myocardial ischemia was induced by exteriorizing the heart with a left thoracic incision followed by a slipknot (5-0 silk) around the left anterior descending coronary artery (LAD). Following 30 min ischemia, the slipknot was released and the animal underwent 120 min reperfusion. The Sham group underwent the same procedure without LAD ligation.

Rats were randomly assigned to one of four groups (n=15): i) A sham group; ii) an MI/R group that received treatment vehicle with 0.9% sodium chloride i.p.; iii) an MI/R+PTB group that received PTB (10 mg/kg, i.p.) 10 min prior to reperfusion iv) and an MI/R+PTB+LY group that received PTB (10 mg/kg, i.p.) 10 min prior to reperfusion and LY (10 mg/kg, i.p.) every 2 days three times prior to surgery.

Evaluation of myocardial infarct size

Tetrazolium chloride (TTC) staining was used to assess the myocardial infarct size. Following reperfusion, rats were anaesthetized and euthanized. Rat hearts were then immediately isolated, washed in PBS and sectioned into transverse slices 5 mm thick. Following incubation in 1% (0.01 g/ml) TTC at 37°C in PBS for 15 min, heart slices were photographed with a digital camera to distinguish between the red-stained viable tissues and the white-unstained infarcted tissues. Areas of infarct size were measured digitally using Image Pro Plus 6.0 software (Media Cybernetics Inc., Rockville, MD, USA).

Evaluation of serum levels of CK-MB and LDH

Following reperfusion, blood (6 ml) was taken from the carotid artery of all rats and kept at room temperature for 30 min. Serum was collected by centrifugation at 3,000 × g at 4 °C for 15 min and kept at −70°C for preservation. The CK-MB and LDH assay kits were used to measure levels of CK-MB and LDH in the serum, following the manufacturer's instructions. Enzyme activity was expressed in U/l.

Assay of MPO activity and levels of the inflammatory cytokines TNF-α, IL-6, IL-8 in the serum

Following reperfusion, the myocardial tissue was kept at −70°C for preservation. An MPO assay kit was used to detect MPO levels in the myocardial tissue following the manufacturer's instructions. Levels of TNF-α, IL-6 and IL-8 were spectrophotometrically analyzed using ELISA kits following the manufacturer's instructions.

Determination of myocardial apoptosis

Myocardial apoptosis was determined using TUNEL staining. The tissue samples were fixed using 4% formaldehyde at 4°C for, 24 h and embedded in paraffin. The paraffin-embedded tissue was cut into sections 4–5 µm thick. Sections were incubated in 50 µl TUNEL mixture (solution A: Solution B=1:9) in a humidified chamber at 37°C for 1 h. Slides were incubated with DAPI for 5 min at room temperature in the dark to detect the nuclei. Sections were covered using cover slips with anti-fade mounting medium (cat. no. P0126; Beyotime Institute of Biotechnology, Haimen, China). Sections were observed using fluorescence microscopy in six random fields of view. The apoptotic index was calculated as the ratio of the number of TUNEL-positive neurons to the total number of nuclei.

Western blot analysis

Heart tissues were lysed with RIPA lysis buffer (cat. no. sc-24948, Santa Cruz Biotechnology, Inc., Dallas, TX, USA). Following sonication at 4°C for 25 sec at a frequency of 20 kHz, lysates were centrifuged at 14,000 × g at 4°C for 30 min and protein concentration was detected using the BCA determination method. Proteins (30 µg/lane) were then separated by SDS-PAGE (8–15%) and transferred to a polyvinylidene difluoride membrane (EMD Millipore, Billerica, MA, USA). Following blocking with 5% skimmed milk in Tris-buffered saline at room temperature for 1 h, the membrane was incubated with primary antibodies against p-Akt (1:1,000), Akt (1:1,000), Bcl-2 (1:1,000), Bax (1:1,000) and β-actin (1:1,000) overnight at 4°C. The membrane was then washed with PBS and incubated with horseradish peroxidase-conjugated immunoglobulin G secondary antibody (1:10,000) for 1 h at 37°C. Blots were developed using a SuperSignal chemiluminescence detection kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and immunoblotting was visualized and analyzed using the Quantity One System 4.62 (Bio-Rad, Inc., Hercules, CA, USA).

Statistical analysis

Data are presented as the mean ± standard error of the mean. SPSS 18.0 (SPSS, Inc., Chicago, IL, USA) was used to perform statistical analysis. Differences among groups were evaluated by one-way analysis of variance followed by a Dunnett's t-test for multiple comparisons. P<0.05 was determined to indicate a statistically significant difference.

Results

PTB decreases myocardial infarct size via PI3K/Akt signaling pathway activation

A myocardial infarct was induced all groups apart from the Sham group (Fig. 1). Treatment with PTB significantly decreased the myocardial infarct size compared with the M/IR group (P<0.05; Fig. 1). However, the protective effect of PTB was reversed by LY treatment, as the myocardial infarct size was significantly increased in the MI/R+PTB+LY group compared with the MI/R+PTB group (P<0.05; Fig. 1).

PTB decreases the release of myocardial CK-MB and LDH following MI/R via PI3K/Akt signaling pathway activation

The release of CK-MB and LDH is an indicator of myocardial injury; therefore, serum levels of CK-MB and LDH were measured. There was a significant increase in the release of serum CK-MB and LDH in the MI/R group compared with the Sham group (both P<0.05; Fig. 2). However, there was a significant decrease in the release of CK-MB and LDH in the MI/R+PTB group compared with the MI/R group (both P<0.05; Fig. 2). The release of CK-MB and LDH was significantly increased in the MI/R+PTB+LY group compared with the MI/R+PTB group (P<0.05; Fig. 2) indicating that PI3K was involved in the protective effect of PTB against MI/R injury.

PTB decreases myocardial MPO activity and the serum levels of TNF-α, IL-6 and IL-8 following MI/R via activation of the PI3K/Akt signaling pathway

Increased MPO activity in the myocardium indicates neutrophil infiltration. MPO activity in the MI/R group was significantly increased compared with the Sham group (P<0.05; Fig. 3A). However, PTB significantly decreased myocardial MPO activity in the MI/R+PTB group compared with the MI/R group and LY treatment reversed the effect of PTB (both P<0.05; Fig. 3A). Levels of TNF-α, IL-6, and IL-8 in the plasma were all significantly increased following MI/R injury compared with the Sham group (all P<0.05; Fig. 3B-D). Treatment with PTB significantly decreased cytokine levels in the serum (all P<0.05; Fig. 3B-D) and these effects were reversed following LY treatment (all P<0.05; Fig. 3B-D).

PTB reduces myocardial apoptosis induced by MI/R via activation of the PI3K/Akt signaling pathway

TUNEL staining was performed to evaluate apoptosis in the rat hearts (Fig. 4). The apoptotic index of the MI/R+PTB group was significantly decreased compared with the MI/R group (P<0.05; Fig. 4). However, treatment with LY again significantly reversed the protective effects of PTB (P<0.05; Fig. 4), indicating that PI3K activation is involved in the mechanism of action of PTB against MI/R-induced myocardial apoptosis.

Effects of PTB on the expression of p-Akt, Akt, Bcl-2 and Bax in hearts subjected to MI/R

MI/R injury in the MI/R group significantly increased the phosphorylation of Akt compared with the Sham group (P<0.05; Fig. 5A and B). The phosphorylation of Akt was further increased following treatment with PTB (P<0.05, Fig. 5A and B). However, treatment with LY reversed the increase in Akt phosphorylation that was induced by PTB (P<0.05; Fig. 5A and B). In addition, MI/R injury significantly decreased Bcl-2 expression in the MI/R group compared with the Sham group (P<0.05; Fig. 5C). Treatment with PTB significantly increased the expression of Bcl-2 (P<0.05), which was subsequently decreased by co-administration with LY (both P<0.05; Fig. 5C). By contrast, MI/R injury induced a significant increase in Bax expression compared with the Sham group (P<0.05; Fig. 5D). PTB administration significantly decreased the expression of Bax in the MI/R group compared with the MI/R+PTB group (P<0.05), which was significantly reversed by co-administration with LY (both P<0.05; Fig. 5D).

Discussion

The current study demonstrated that myocardial I/R injury is attenuated by treatment with PTB. PTB decreased cardiac injury, alleviated myocardial apoptosis and decreased the inflammation induced by myocardial I/R injury. The protective effect of PTB is strongly associated with activation of the PI3K/Akt signaling pathway. Treatment with PTB significantly decreased the release of CK-MB and LDH and the activity of MPO following myocardial I/R injury. PTB administration also decreased levels of TNF-α, IL-6, and IL-8 in the serum. Furthermore, treatment with PTB reduced myocardial apoptosis. However, the cardioprotective effects of PTB were reversed following treatment with the PI3K signaling inhibitor LY, suggesting that activation of the PI3K/Akt signaling is involved in the cardioprotective effects of PTB.

CK is an enzyme expressed in various types of tissues and its isoenzyme is CK-MB in the myocardium. LDH is an enzyme that catalyzes the conversion of lactate to pyruvic acid. The isoenzyme of LDH in the heart is LDH-1 and may be used to identify damage in the heart (13). Following cardiomyocyte damage, cellular CK-MB and LDH are released into the blood, resulting in increased levels of CK-MB and LDH in the serum. The results of the present study demonstrate that the serum levels of CK-MB and LDH are decreased following PTB treatment, suggesting that PTB treatment alleviates myocardial injury following I/R.

MPO is a peroxidase enzyme, which is most abundantly expressed in neutrophils. Therefore, MPO activity in the myocardium is regarded as an indicator of neutrophil infiltration and inflammation. The current study demonstrated that PTB treatment decreased MPO activity in the myocardium, suggesting that PTB may attenuate neutrophil infiltration and the inflammatory response following MI/R injury. Inflammation serves an important role in MI/R injury. Damaged cells stimulate inflammatory cells, which subsequently release various inflammatory cytokines. The release of cytokines causes further injury to endothelial cells, resulting in increased vascular permeability (14). Therefore, in the current study, serum levels of TNF-α, IL-6, and IL-8 were measured to determine the anti-inflammatory effect of PTB. The results demonstrated that PTB treatment deceases serum levels of TNF-α, IL-6, and IL-8. Overall, these results demonstrate that PTB decreases neutrophil infiltration and the inflammatory response during myocardial I/R injury.

Myocardial apoptosis also serves an important role in myocardial I/R injury. It is inhibited by Bcl-2 and induced by Bax (15). The effect of PTB on myocardial apoptosis was assessed using TUNEL staining in the current study. The results indicate that PTB treatment reduces the number of TUNEL-positive cells. Furthermore, levels of Bcl-2 and Bax were measured using western blotting and the results demonstrated that PTB treatment increases Bcl-2 expression and decreases Bax expression. However, the protective effects of PTB were inhibited by LY, a PI3K signaling inhibitor.

PI3K/Akt signaling is strongly associated with cell survival (16,17). Previous studies have demonstrated that PI3K/Akt signaling confers significant cardioprotective effects (18,19). Akt phosphorylation suppresses apoptosis and promotes cell survival in myocardial ischemia (2022). The results of the current study demonstrate that PTB treatment upregulates Akt phosphorylation, indicating that the protective effects of PTB are associated with activation of the PI3K/Akt signaling pathway. Treatment with LY reverses the increase in Akt phosphorylation, further confirming this conclusion.

In conclusion, the present study demonstrates that PTB, an antioxidant and anti-inflammatory agent, attenuates myocardial I/R injury. The results also indicate that the activation of the PI3K/Akt signaling pathway by PTB protects against myocardial I/R injury. Thus, these findings may facilitate the development of PTB as a therapeutic strategy to treat myocardial I/R injury.

References

1 

Ramzy D, Rao V and Weisel RD: Clinical applicability of preconditioning and postconditioning: The cardiothoracic surgeons's view. Cardiovasc Res. 70:174–180. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Yue Y, Yang X, Feng K, Wang L, Hou J, Mei B, Qin H, Liang M, Chen G and Wu Z: M2b macrophages reduce early reperfusion injury after myocardial ischemia in mice: A predominant role of inhibiting apoptosis via A20. Int J Cardiol. July 26–2017.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI

3 

Zhang Y, Wang XL, Zhao J, Wang YJ, Lau WB, Yuan YX, Gao EH, Koch WJ and Ma XL: Adiponectin inhibits oxidative/nitrative stress during myocardial ischemia and reperfusion via PKA signaling. Am J Physiol Endocrinol Metab. 305:E1436–E1443. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Shimizu Y, Lambert JP, Nicholson CK, Kim JJ, Wolfson DW, Cho HC, Husain A, Naqvi N, Chin LS, Li L and Calvert JW: DJ-1 protects the heart against ischemia-reperfusion injury by regulating mitochondrial fission. J Mol Cell Cardiol. 97:56–66. 2016. View Article : Google Scholar : PubMed/NCBI

5 

McCormack D and McFadden D: A review of pterostilbene antioxidant activity and disease modification. Oxid Med Cell Longev. 2013:5754822013. View Article : Google Scholar : PubMed/NCBI

6 

Bhakkiyalakshmi E, Sireesh D, Sakthivadivel M, Sivasubramanian S, Gunasekaran P and Ramkumar KM: Anti-hyperlipidemic and anti-peroxidative role of pterostilbene via Nrf2 signaling in experimental diabetes. Eur J Pharmacol. 777:9–16. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Guo Y, Zhang L, Li F, Hu CP and Zhang Z: Restoration of sirt1 function by pterostilbene attenuates hypoxia-reoxygenation injury in cardiomyocytes. Eur J Pharmacol. 776:26–33. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Wang B, Liu H, Yue L, Li X, Zhao L, Yang X, Wang X, Yang Y and Qu Y: Neuroprotective effects of pterostilbene against oxidative stress injury: Involvement of nuclear factor erythroid 2-related factor 2 pathway. Brain Res. 1643:70–79. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Fruman DA and Cantley LC: Phosphoinositide 3-kinase in immunological systems. Semin Immunol. 14:7–18. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Cantley LC: The phosphoinositide 3-kinase pathway. Science. 296:1655–1657. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Fujio Y, Nguyen T, Wencker D, Kitsis RN and Walsh K: Akt promotes survival of cardiomyocytes in vitro and protects against ischemia-reperfusion injury in mouse heart. Circulation. 101:660–667. 2000. View Article : Google Scholar : PubMed/NCBI

12 

National Research Council, . Guide for the Care and Use of Laboratory Animals. 8th edition. National Academies Press (US); Washington, DC: 2011, PubMed/NCBI

13 

Li GH, Luo B, Lv YX, Zheng F, Wang L, Wei MX, Li XY, Zhang L, Wang JN, Chen SY, et al: Dual effects of VEGF-B on activating cardiomyocytes and cardiac stem cells to protect the heart against short- and long-term ischemia-reperfusion injury. J Transl Med. 14:1162016. View Article : Google Scholar : PubMed/NCBI

14 

Lefer AM, Ma XL, Weyrich A and Lefer DJ: Endothelial dysfunction and neutrophil adherence as critical events in the development of reperfusion injury. Agents Actions Suppl. 41:127–135. 1993.PubMed/NCBI

15 

Niture SK and Jaiswal AK: INrf2 (Keap1) targets Bcl-2 degradation and controls cellular apoptosis. Cell Death Differ. 18:439–451. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Armstrong SC: Protein kinase activation and myocardial ischemia/reperfusion injury. Cardiovasc Res. 61:427–436. 2004. View Article : Google Scholar : PubMed/NCBI

17 

He F, Xu BL, Chen C, Jia HJ, Wu JX, Wang XC, Sheng JL, Huang L and Cheng J: Methylophiopogonanone A suppresses ischemia/reperfusion-induced myocardial apoptosis in mice via activating PI3K/Akt/eNOS signaling pathway. Acta Pharmacol Sin. 37:763–771. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Li H, Song F, Duan LR, Sheng JJ, Xie YH, Yang Q, Chen Y, Dong QQ, Zhang BL and Wang SW: Paeonol and danshensu combination attenuates apoptosis in myocardial infarcted rats by inhibiting oxidative stress: Roles of Nrf2/HO-1 and PI3K/Akt pathway. Sci Rep. 6:236932016. View Article : Google Scholar : PubMed/NCBI

19 

Fang J, Hu F, Ke D, Yan Y, Liao Z, Yuan X, Wu L, Jiang Q and Chen L: N,N-dimethylsphingosine attenuates myocardial ischemia-reperfusion injury by recruiting regulatory T cells through PI3K/Akt pathway in mice. Basic Res Cardiol. 111:322016. View Article : Google Scholar : PubMed/NCBI

20 

Mullonkal CJ and Toledo-Pereyra LH: Akt in ischemia and reperfusion. J Invest Surg. 20:195–203. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Pei YH, Chen J, Xie L, Cai XM, Yang RH, Wang X and Gong JB: Hydroxytyrosol protects against myocardial pschemia/reperfusion injury through a PI3K/Akt-dependent mechanism. Mediators Inflamm. 2016:12321032016. View Article : Google Scholar : PubMed/NCBI

22 

Sun Y, Ye L, Jiang C, Jiang J, Hong H and Qiu L: Over-expression of HSPA12B protects mice against myocardium ischemic/reperfusion injury through a PPARγ-dependent PI3K/Akt/eNOS pathway. Am J Transl Res. 7:2724–2737. 2015.PubMed/NCBI

Related Articles

Journal Cover

December-2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Hu L, Cai N and Jia H: Pterostilbene attenuates myocardial ischemia‑reperfusion injury via the phosphatidylinositol 3'‑kinase‑protein kinase B signaling pathway. Exp Ther Med 14: 5509-5514, 2017
APA
Hu, L., Cai, N., & Jia, H. (2017). Pterostilbene attenuates myocardial ischemia‑reperfusion injury via the phosphatidylinositol 3'‑kinase‑protein kinase B signaling pathway. Experimental and Therapeutic Medicine, 14, 5509-5514. https://doi.org/10.3892/etm.2017.5246
MLA
Hu, L., Cai, N., Jia, H."Pterostilbene attenuates myocardial ischemia‑reperfusion injury via the phosphatidylinositol 3'‑kinase‑protein kinase B signaling pathway". Experimental and Therapeutic Medicine 14.6 (2017): 5509-5514.
Chicago
Hu, L., Cai, N., Jia, H."Pterostilbene attenuates myocardial ischemia‑reperfusion injury via the phosphatidylinositol 3'‑kinase‑protein kinase B signaling pathway". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5509-5514. https://doi.org/10.3892/etm.2017.5246